Diversity among 2481 Escherichia coli from women with community-acquired lower urinary tract infections in 17 countries (ECOSENS project)
Abstract number: 902_p1101
In the ECO.SENS project the prevalence and susceptibility of pathogens causing community-acquired acute uncomplicated urinary tract infections in women in 16 European countries and Canada was investigated (JAC 2003; 51: 6976). It revealed vast differences in resistance between countries. We used the PhenePlateTM (PhP) System to determine the clonal diversity of the 2481 E. coli of the ECO.SENS survey.
The E. coli were typed using the PhP system. This is a biochemical typing method which measures not only positive and negative reactions but also the kinetics of each reaction. PhP-RE plates, specifically intended for use with E. coli, include 11 reagents. The speed and result of the reactions (measured at 8, 24 and 48 h of incubation) are expressed in a numerical code. Data were analysed with the PhPWIN Software.
The PhP system divided the 2481 E. coli strains into 74 Common PhP Types (CT), each containing two or more isolates (n = 2067), and 414 Single PhP Types. The diversity index was 0.94 among all isolates. The antimicrobial susceptibility patterns identified 46 types and yielded a diversity of 0.55 among all isolates. Type CT-48 was the most frequent type in 13 countries and the second most frequent in four countries. It contained 400 isolates and exhibited 11 different antimicrobial resistance patterns. Sixty-four per cent of the E. coli isolates did not exhibit resistance to any of the investigated drugs. Frequent susceptibility patterns were isolated resistance to ampicillin, combined resistance to ampicillin and trimethoprim, single resistance to trimethoprim followed by single resistance to nalidixic acid.
Although some types exhibited distinct differences in their susceptibility patterns there was no obvious correlation between the phenotypes identified with the PhP system and the susceptibility pattern. Thus, our data did not suggest a dissemination of resistant clones within or between countries as an explanation for differences in antimicrobial resistance rates."
|Session name:||XXIst ISTH Congress|
|Back to top|