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High prevalence of CTX-M type extended-spectrum beta-lactamases in members of Enterobacteriaceae in Turkey

Abstract number: 902_p752

Gulay Z.

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Objective:

To find the prevalance and predominant types of CTX-M type beta-lactamases in Enterobacteriaceae isolated in different regions of Turkey.

Methods:

Seventy clinical isolates recovered between November 2002 and July 2003 at seven medical centres located in six cities, were taken into the study. Isolates were chosen randomly on a 10 isolate per centre basis. The isolates consisted of 34 Escherichia coli, 23 Klebsiella pneumoniae, eight Enterobacter spp., two K. oxytoca, two Proteus mirabilis and one Shigella sonnei. All isolates were extended spectrum beta-lactamase (ESBL) producers according to the NCCLS criteria and confirmatory tests. B-lactamases were characterised by PCR amplification using specific primers. Conjugation experiments and isoelectric focusing was also performed. PCR products of 20 isolates (two to three isolates per centre) were sequenced in both strands.

Results:

The prevalance of CTX-M type beta-lactamases was 50–100% at different centres. Enzyme production was detected in 76.5, 82.6 and 50% of E. coli, K. pneumoniae and Enterobacter isolates, respectively. One K. oxytoca and the Shigella isolate also produced CTX-M type ESBL(s). None of the P. mirabilis isolates were CTX-M producers. The beta-lactamases were transferred to the recipient E. coli K-12 (SmR) from 66% of the donors. Sequencing results revealed CTX-M-3 production in all isolates.

Conclusion:

This study shows that CTX-M type enzymes and particularly CTX-M-3 is widely disseminated in microorganisms belonging to the family of Enterobacteriaceae in Turkey.

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Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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