Aim:

the aim was to record the field potentials which are an electrical phenomenon produced by neurons and muscle cells. The signals were recorded using a low impedance extracellular microelectrode.

Methods:

field potentials investigated Electrophysiological using extracellular microelectrodes. Transverse slices of 500µm thickness prepared from the hippocampus of adult Wistar rats were incubated for at least 60 min in an artificial cerebrospinal fluid (CSF) of the following composition (mM): NaCI 124, KCl 3, MgS0₄ 1.25, CaCl₂ 2.5, KH₂P0₄ 1.25, NaHCO) 22, Glucose 10. The solution was continuously gassed at 33°C with a mixture of 95% O₂ and 5% CO₂ to attain a steady-state oxygenation level and maintain a pH of 7.4, The slices were super fused at I ml/min in a narrow chamber. Half-maximal population spikes were recorded in stratum pyramidal of area CA1 as averages of 8 sweeps. Recording electrodes were glass micropipettes filled with I M NaCI (resistance 8-10 MW). Orthodromic stimulation of the Schaffer collateral pathway was performed at a frequency of 0.1 Hz (100 msec pulses) using a twisted pair of Teflon coated wires. All drugs i.e. the extract of plant Hyocyamus reticulates L. from the Solanaceae family was purified in pharmacology department. The rate of yield was 15.63 % (15,63g extract from 100g Plant). The extraction was solved in DMSO (dimethyl sulfoxide, Sigma-Aldrich Chemie Gmbh, German) and diluted in artificial cerebrospinal fluid at various concentrations.

Results:

The effects of Bad-Bad? extract was recorded on the extracellular field potentials of the hippocampus pyramidal cell area CA1.Field potentials were recorded during the effect of the extract and -non extract. Amplitude of the field potentials decreased 20% when the extract was diluted ten times (n=6). The amplitude of field potentials decreased 40 % when the extract was diluted hundred times (n=6). And, the amplitude of field potentials decreased 40 % when the extract was diluted two hundred times (n=6). After every processing the application areas of the bad-bad? were washed out, carefully.

Conclusions:

Results of many researches has shown that hippocampus CA1 area has important function for learning and memory. It was known that hippocampus prepare specific electrical events as related learning and memory. The effective substances in Bad-Bad? extract can inhibit impulse on this area in specific doses and thus, other impulses can pass easily to brain cortex. This hypothesis support that hippocampus have active function as related memory process.

This study was supported by Scientific Research Projects (2003: YTR-002) of Yuzüncü Yil Uni., Van, Turkey