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Acta Physiologica Congress

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Acta Physiologica 2012; Volume 206, Supplement 692
The 63rd National Congress of the Italian Physiological Society
9/21/2012-9/23/2012
Verona, Italy


ACTIVATION OF VEGF PATHWAYS ALTERS COPPER TRAFFICKING IN HUMAN ENDOTHELIAL CELLS
Abstract number: P4.36

URSO1 E, RIZZELLO A, DE RICCARDIS1 L, DANIELI1 A, ACIERNO1 R, MAFFIA1 M

1DeptEnvironmental and Biological Sciences and Technologies, Salento Univ., Lecce, Italy

Neovascularization is severely affected by a copper (Cu)-deficient milieu. Several pro-angiogenic cytokines are induced (VEGF), activated (angiogenin) or efficiently secreted (FGF-1) by Cu-dependent pathways. Conversely, some factors involved in the control of angiogenic events (i.e. VEGF, PDGF) induce the intracellular movement of perinuclear Cu stores toward the edge of cytoplasmic projections in migrating blood vessel cells. Consensus has not been achieved about the identity and the physiological behaviour of proteins involved in the cell Cu trafficking during angiogenesis. To shed light on this aspect, the Cu-dependent effects of the best-known pro-angiogenic factor VEGF have been analysed in a human primary macrovascular cell model (HUVEC). Preliminarily, the VEGF proliferative and chemotactic potency has been shown to be significantly reduced by the administration of Cu chelating agents. In addition, VEGF stimulation has been found to promote the translocation of the ATP7A Cu efflux pump toward the cell periphery (confocal microscopy). Such redistribution was associated with an increased release of Cu into the extracellular medium (spectrofluorimetric techniques). In agreement, the expression profile of Cu transport proteins in response to VEGF revealed that the Cu secretory pathway was preferentially activated, that providing a mechanism to amplify the angiogenic response through the secretion of proteolytic cuproenzymes enabling the endothelial invasiveness.

To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 206, Supplement 692 :P4.36

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