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Acta Physiologica 2012; Volume 206, Supplement 692
The 63rd National Congress of the Italian Physiological Society
9/21/2012-9/23/2012
Verona, Italy
POST-TRANSLATIONAL MODIFICATIONS (PTM) AND PROTEIN TRANSLOCATION INTO MITOCHONDRIA (MITO) AFTER ISCHEMIA-REPERFUSION (I-R) AND INDIRECT (ISCHEMIC POSTCONDITIONING, I-POSTC) OR DIRECT (PHARMACOLOGICAL POSTC, P-POSTC) TARGETING OF MITO
Abstract number: P4.29
PERRELLI1 M-G, TULLIO1 F, ANGOTTI1 C, PAGLIARO1 P, PENNA1 C
1Dept Scienze Cliniche e Biologiche, Univ. di Torino, Italy
I-PostC or P-PostC in the early reperfusion phase can limit I-R injury. The I-PostC favors NO and redox signaling with S-nitrosylation (SNO) of proteins and phosphorylation (Phospho) of RISK and SAFE elements, which converge on MITO. Whether P-PostC induces a similar signaling modulation and protein translocation within MITO is unknown. We compared the effects of I-PostC and P-PostC on RISK and SAFE pathways, and markers of apoptosis and autophagy in cytosolic (CF) and mitochondrial (MF) fractions. Isolated rat hearts underwent 1) Sham perfusion; 2) 30-min I plus 2-hours R; 3) I-PostC (5 intermittent cycles of 10-s R and 10-s I immediately after the 30-min I), or 4) P-PostC (infusion of Diazoxide in the first 3-min of 2-h R). Phospho and SNO of proteins were studied by Western blotting and biotin switch assay, respectively. Both I-PostC and P-PostC induced a significant Phospho of elements of RISK and SAFE; in particular Phospho-Akt and Phospho-STAT3 were transolocated into the MITO. On the contrary, Phospho-PKCd appears mainly located in CF and MF during I-R than after I-PostC or P-PostC. Cleaved caspase-3 (index of apoptosis) and beclin-1 (index of autophagy) levels were reduced by either I-PostC or P-PostC. Both maneuvers increased the levels of SNO of proteins within MF.
Indirect (I-PostC) or direct (P-PostC) targeting of MITO activate similar pathways (RISK and SAFE), PTM (Phospho and SNO) and translocation of proteins; thus decreasing apoptosis and autophagic markers.
To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 206, Supplement 692 :P4.29