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Acta Physiologica 2012; Volume 206, Supplement 692
The 63rd National Congress of the Italian Physiological Society
9/21/2012-9/23/2012
Verona, Italy
IDENTIFICATION OF HUMAN RETINAL PIGMENT EPITHELIUM (HRPE) CELLS AS A VALUABLE MODEL TO STUDY EPITHELIAL AND NEURONAL POLARIZATION.
Abstract number: P4.28
PAVAN1 B, DALPIAZ2 A, PAGANETTO1 G, CAPUZZO1 A
1Dept of Biology, Ferrara Univ., Ferrara, Italy
2Dept of Pharmaceutical Sciences, Ferrara Univ., Ferrara, Italy
Retinal pigment epithelial cells and dopamine retinal neurons share the same original precursor cells. It is meaningful to explore the differentiation potential of the established HRPE cell line into retinal neurons or epithelial cell monolayer showing polarized barrier features. To improve the culture environment for the HRPE cells epithelial polarization, Millicell filter inserts were applied, feeding the cells with media of different composition at the apical and basolateral sides. This arrangement allowed the cells to acquire directional polarization, as demonstrated by transepithelial electrical resistance measurements (80±7 Wcm2). This system provided a useful tool for investigating blood-central nervous system barriers functions. Furthermore, we observed that factors such as retinoic acid, epidermal and basic fibroblast growth factors were able to convert HRPE cells into neuron-like cells. However, since such treatment did not actually transform these cells into mature neurons, we investigated the efficacy of other factors in these differentiation-competent cells using light microscopy, ELISA, and RIA assays. We have preliminary noted that a storage of dopamine was induced in differentiation-cocktail treated unlike the untreated cells. Moreover, we characterized the expression and regulation of dopamine-sensitive adenylyl cyclases (AC) specific isoforms, such as AC5 and AC6, using stimulating and/or inhibiting selective compounds on AC activity.
To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 206, Supplement 692 :P4.28