The consolidation of changes following activity-dependent neural plasticity are believed to involve specific patterns of gene expression. In the hippocampus, immediate early genes are thought to contribute to long-term synaptic plasticity (LTP and LTD);

Immediate early genes are thought to contribute to long-term synaptic plasticity (LTP and LTD) in the hippocampus. We employed VSD imaging in rat cerebellar slices in order to map LTP/LTD spatial distribution in the cerebellum granular layer at 15' and 120' following a Theta Burst Stimulus delivered to the mossy fibers. Slices were then fixed and processed for immunohistochemistry and in situ hybridization in order to identify c-Fos and P-CREB expression patterns at the protein and mRNA level. The induction of long-term plasticity increased the average level of P-CREB both at 15' and 120' after TBS, while c-Fos was unaltered at 15' and significantly increased at 120'M APV showed that, in correspondence with the block of LTP and LTD induction, c-Fos and P-CREB levels were unchanged, confirming their involvement in cerebellar plasticity.(. By spatially correlating long-term synaptic plasticity with the corresponding variation of P-CREB and c-Fos, we observed that regions showing LTP well correlated with positive variations of P-CREB and c-Fos. Conversely, areas showing LTD correlated exclu-sively with negative variations of P-CREB. The analysis performed in the presence of 50