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Acta Physiologica 2012; Volume 206, Supplement 693
Joint FEPS and Spanish Physiological Society Scientific Congress 2012
9/8/2012-9/11/2012
Santiago de Compostela, Spain
EFFECTS OF EXTRACTS OBTAINED FROM WINE BY-PRODUCTS ON ISOLATED RAT DUODENUM
Abstract number: P71
Valero1 MS, Lopez1 V, Coronado1 V, Berzosa1 C, Gomez-Rincon1 C, Mainar2 AM, Murillo3 MD, Langa1 E
1Ciencias de la Salud, Universidad San Jorge,
2Group of Applied Thermodynamics and Surfaces (GATHERS), Instituto de Investigacin en Ingeniera de Aragn (I3A),, Universidad de Zaragoza,
3Fisiologa y Farmacologa, Universidad San Jorge
Objectives:
Study the biological effects of extracts obtained from the wine process wastes in the smooth muscle of rat duodenum and in an in vitro antioxidant capacity.
Materials:
Some solvent-free extracts obtained from the wastes of the wine process were tested as vasorelaxants on rat duodenum. These extracts were obtained by the Group of Applied Thermodynamics and Surfaces (GATHERS) and in order to diversify the composition of the extracts several extraction parameters were modified when Soxhlet technique was applied. The extracts were dissolved in a DMSO/H2O solution. Piece of male rats duodenum were cut into small segments, suspended in the direction of smooth muscle fibres in a thermostatically controlled organ bath containing Krebs solution at 37ordm;C to reach pH 7.4, continuously gassed with 95% O2 and 5% CO2. Segments were stretched passively into an initial tension of 20mN. Each segment was connected to an isometric transducer and mechanical activities were recorded, stored and analyzed using the MacLab Systems software. The extracts were added to organ bath to study the effects on duodenal spontaneous contractions and on the contractions induced by KCl (80mM). The antioxidant activity was studied through the DPPH photometric assay in 96-well microplates using ascorbic acid as positive control. Statistical analyses were determined using a paired t-test, with a level of significance p<0.05.
Results:
The extracts induced relaxant responses on spontaneous contractions of longitudinal smooth muscle of rat duodenum and reduced the contractions induced by KCl 80mM. The relaxant responses of extracts were compared respect to papaverine 0.1mM or Ca2+ free Krebs with EGTA 2mM.Two extracts (out of five) exerted antioxidant properties in the DPPH antioxidant assay with the following IC50 values: 115.5 and 97.4mg/ml.
Conclusions:
Extracts obtained from the wine process wastes showed relaxant effect on longitudinal smooth muscle of duodenum and in vitro antioxidant activity which means that may have healthy properties.
To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 206, Supplement 693 :P71