Objectives: 

Probiotics which are living microorganisms that enter the gastrointestinal tract and prevent of adhesion of pathogen microorganisms by competition in intestine. There are many studies that show probiotics have affirmative effects on many gastrointestinal disorder. We aim to understand the probiotics mediated regulation in the gastrointestinal system more clearly.

Materials: 

Sprague-Dawley rats(250-280 g) feed with most common used probiotics S. Cerevisiae(10 mg/rat/day) or S. Boulardii (10 mg/rat/day) in 2ml tap water by intragastric gavage, control groups only take same volume tap water . Probiotics given 1 hour before the decapitation(acute)or 14 days(chronically).

At the end of the experiment gastric and intestinal and colonic tissues removed for study of malondialdehyde(MDA), glutathione(GSH) levels and myeloperoxidase(MPO) activity. Values are means ± S.E.M compared by ANOVA.

Results: 

Acute treatment of probiotics not significantly changed tissue MPO, MDA, and GSH levels. MPO levels significantly decreased with S.Cerevisiae(p<0.01) and increased with S.Boulardii in all intestinal tract. Acute treatment of probiotics not significantly changed tissue MDA and GSH levels. Chronic treatment of S.Cerevisiae not significantly changed the MDA and GSH levels but significantly changed with S. Boulardii(p>0.05-p<0.01).

Conclusions: 

We investigated that both of two probiotics exhaust the antioxidant levels and S. Boulardii increased MPO activity in chronic condition. We conclude that probiotics diet modulates gastrointestinal redox status depending on duration of use, and chronic treatments may cause immunologic responses.