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Acta Physiologica 2012; Volume 205, Supplement 690
Joint Meeting of the Hungarian Biophysical Society, Hungarian Physiological Society, Hungarian Society of Anatomists and Hungarian Society of Microcirculation & Vascular Biology
6/11/2012-6/13/2012
Debrecen, Hungary
THE EFFECT OF RECREATIONAL PHYSICAL EXERCISE ON THE ACTIVITY AND EXPRESSION OF HEME OXYGENASE, NITRIC OXIDE SYNTHASE AND MATRIX METALLOPROTEINASE ENZYMES IN RAT CARDIOVASCULAR SYSTEM
Abstract number: P44
Szabo1 R, Posa1 A, Magyarine1 BA, Szalai1 Z, Menesi1 R, Varga1 Cs
1Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Budapest
Introduction:
Physical inactivity, i.e. sedentary life style increases the development of the risk factors of circulatory disease such as: high blood pressure, diabetes, the lipid metabolism and obesity. Carbon monoxide and nitric oxide produced by the heme oxygenase (HO) and by the constitutive nitric oxide synthase (cNOS) protect circulation. In contrast, the enhanced level of the matrix metalloproteinase (MMP) augments cardiovascular risks.
Design:
Male Wistar rats were placed into cages installed with running wheels allowing them the self administration of physical exercise over 6 weeks. We studied 1. the activity and expression of HO and NOS enzymes in the aorta and heart left ventricle (LV); 2. the serum level of MMP-2; 3. the angina susceptibility of the heart (assessed by lead II. surface ECG following adrenaline plus phentolamine challenge); 4. the infarct size was measured in isolated hearts from both groups (control, trained) and subjected to 30 min coronary occlusion followed by 120 min reperfusion.
Results:
We found that physical exercise 1. increased LV and aortic HO activity (from 0.83±0.21 to 5.35±0.36 and from 0.73±0.15 to 1.60±1.13 nmol bilirubin/h/mg protein, respectively; n=12–15; p<0.001) and LV HO-1 isoenzyme expression (from 106.3 ±3.132 to 164.0±16.479%; n=6–7; p<0.05) and increased LV and aortic cNOS activity (from 16.32±3.71 to 59.11±7.94 and from 105.38±55.72 to 181.78±30.29 pmol/min/mg protein, respectively; n=9–15; p<0.001) and LV endothelial NOS isoenzyme expression (from 109.15±5.247 to 163.1±10.67%; n=3; p<0.05); and 2. decreased MMP-2 serum level (64 KDa; from 1002.71±37.50 to 679.73±34.35 intensity x mm2; n=12–13; p<0.001); and 3. decreased heart ischaemia susceptibility (ST segment depression: from -0.14±0.018 to -0.019±0.019 mV; n=11; p<0.001); 4. reduced infarct size (from 51.2±6.86 to 30.17±3.66 %; n=5–6; p<0.001).
Discussion:
Recreational physical exercise protects the heart against angina and decreases the infarct size, which might be associated with the up-regulation of the HO and NOS enzyme systems. The down-regulation of MMP-2 activation contributes to the protection of the vein wall and its flexibility.
Grant supports: SROP 4.2.1./B-09-1/KNOV-210-0005; SROP 4.2.2.-08/1-2008-0006; Bolyai Scholarship (A Posa).
To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 205, Supplement 690 :P44