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Acta Physiologica 2012; Volume 204, Supplement 689
91st Annual Meeting of The German Physiological Society
3/22/2012-3/25/2012
Dresden, Germany
MATHEMATICAL MODEL ANALYSIS OF THE INFLUENCE OF TRPC1 CHANNELS ON DIRECTED MIGRATION OF MURINE NEUTROPHILS
Abstract number: O102
Dieterich1 *P., Lindemann2 O., Schwab2 A.
1Institute of Physiology, TU Dresden, Dresden, Germany
2Institute of Physiology II, University of Mnster, Mnster, Germany
Cellular motility and the ability of cells to sense and react to changes in their environment are of fundamental importance for an efficient immune defense. Directed cell migration induced by chemoattractants triggers complex molecular signaling cascades including the activation of plasma membrane Ca2+ channels of the TRPC family. Chemotactic stimulation thereby impacts on the cellular migration motor and the steering unit. Here, we intend to quantify the influence of TRPC1 channels on these two components applying a mathematical model analysis. Migration was assessed by time-lapse video microscopy of single neutrophils from wildtype (WT) and TRPC1-/- knockout mice under chemotactic stimulation with fMLP or a peritonitis cocktail (PC). The calculation of mean instantaneous velocities shows a small reduction of less than 10% for TRPC1-/- compared to WT neutrophils. Straightness indices of paths for TRPC1-/- cells display 15% and 30% reduction compared to WT under fMLP and PC conditions, respectively. All cell groups show a rather linear drift towards the chemoattractants. The mean drift velocity induced by chemotaxis is reduced for TRPC1-/- from 4.79 mm/min to 3.07 mm/min (fMLP) and from 6.11 mm/min to 4.12 mm/min (PC). However, time-dependent central (drift-free) mean squared displacements of the cell ensembles are quite similar for WT and TRPC1-/- under fMLP and PC indicating that TRPC1 mainly influences the chemotactic direction system. In addition, the application of a fractional stochastic model shows that strong temporal correlations and superdiffusive behavior are maintained for neutrophils under chemotacting conditions and do not depend on TRPC1.
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Acta Physiologica 2012; Volume 204, Supplement 689 :O102