Hyaluronan is synthesized within the cytoplasm and exported through the cell membrane from fibroblasts by the MRP5 transporter into the extracellular matrix. As hyaluronan is a highly charged anion, we hypothesized, that its translocation through the cell membrane is dependent on the membrane potential. Therefore human skin fibroblasts were incubated under different conditions all decreasing the membrane potential by alternating the K⁺ membrane potential: valinomycin served as a K⁺ transport facilitator through the cell membrane into the cells, while increasing extracellular KCl concentrations were used to stimulate K⁺ influx into the cells. In addition, a variety of K⁺ export inhibitors were applied to increase K+ concentration within the cytoplasm. Both membrane potential and hyaluronan export were determined under these different experimental conditions. While hyaluronan export was not correlated with the membrane potential, it was significantly correlated with the K⁺ efflux, irrespective of the individual class of conductive K⁺ channels involved. We therefore propose that concurrent K⁺ efflux is required to neutralize the emerging negative hyaluronan charges to meet the law of electroneutrality.