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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
THE EFFECTS OF CARBOHYDRATE SOLUTION TO ENDOTOXEMIC RATS, ERYTHROCYTE DEFORMABILITY AND PERFUSION OF SPLANCHNIC AREA
Abstract number: PC310
Comu1 Faruk Metin, Deniz2 Turgut, Akpinar1 Badagül
1Department of Physiology, Kirikkale University School of Medicine, Kirikkale, Turkey
2Department of Emergency Medicine, Kirikkale University School of Medicine, Kirikkale, Turkey
3Department of General Surgery, Kirikkale University School of Medicine, Kirikkale, Turkey
Objective:The aim of this study was to investigate effects of oral carbohydrate (maltodextrin) administration on splanchnic circulation and erythrocyte deformability in endotoxemic rats.
Methods:
Forty-eight male Wistar rats (weighing 180200 g) were used in this study. Group SC (n=8) was designated as sham control. Group S (n=10) served as starvation only. Group S+ lipopolysaccharide [LPS] (n=10) was subjected to endotoxemia. Group CH (n=10) received carbohydrate (Preop, 240 mOsm/lt, 12.5% maltodextrin, sodium 50 mg), 0.5 mL in every 4 hours for 48 hours, through orogastric lavage. Group CH+LPS) (n=10) ingested same amount of carbohydrate and was subjected to endotoxemia. Endotoxemia was induced by intraperitoneal injection of 30 mg/kg of LPS. Erythrocyte deformability index was measured using a filtration technique 5 mm in diameter polycarbonate filter with a fixed flow (1.5 ml / min.) Pyloric, ileal, portal and hepatic surfaces were measured by a laser Doppler microvascular perfusion monitor.
Results:
According to control group, (S + LPS) group, deformability was significantly and negatively affected (p <0.05). Deformability of (S + LPS) and (Ch+LPS) group was measured positively affected although there was no significant difference. LPS caused a significant decrease in hepatic surface blood flow (p<0.05). Ingestion of carbohydrate significantly improved (p<0.05) ileal, pyloric blood flows in endotoxemic rats. Increases in hepatic surface blood flow were found in S and Ch groups, although these changes were not statistically significant.
Conclusions:
These results have shown that endotoxemia caused a significant decrease in splanchnic blood flow. We suggest that decreased blood flow with an increase in bacterial translocation can cause infection development in the gastrointestinal system.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC310