Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
APELIN INDUCES CONTRACTIONS IN RAT MYOMETRIUM IN A CALCIUM FREE CONDITION
Abstract number: PC297
Ercan1 Zubeyde, Kacar1 Emine, Ozcan2 Mete, Canpolat3 Sinan, Kutlu1 Selim, Ayar3 Ahmet
1Firat University, Faculty of Medicine, Department of Physiology, Elaz, TURKEY
2Firat University, Faculty of Medicine, Department of Biophysics, Elaz, TURKEY
3Karadeniz Technical University, Faculty of Medicine, Department of Physiology, Trabzon, TURKEY
Apelin is a novel peptide hormone related to some metabolic and physiological processes including reproductive system. We have previously demonstrated for the first time that apelin has a stimulatory effect on myometrial contractility in rats. In this study, we investigated possible stimulatory effect of apelin on myometrial contractility in calcium free condition.
Adult Wistar rats in dioestrus were decapitated and myometrium strips were removed (n=6). All strips were placed in a jacked tissue bath containing Krebs solution at 37 oC and pH 7.4, constantly bubbled with 95% oxygen and 5% carbon dioxide and were allowed to contract under 1g tension and isometric contractions were measured by force displacement transducer.
After equlibration of spontaneous contractions, calcium free condition was set and contractions were disappeared immediately because of lack of extracellular calcium source. This silent period (no contraction) was recorded for 20 mins and apelin was added to the organ bath. Apelin was induced myometrial contraction at 10uM concentration in calcium free medium.
According to the results of this study and our previous data, apelin can stimulate uterus contractions by inducing calcium release from intracellular storages. Further detailed investigations are needed to clarify the mechanism(s) of apelin induction on myometrium contractility and the role of apelin in pregnancy and parturition processes.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC297