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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
THE EFFECTS OF PDE5 INHIBITORY DRUGS ON RENAL ISCHEMIA/REPERFUSION INJURY IN RATS
Abstract number: PC290
Kucuk1 Ay[scedil]egul, Yucel2 Mehmet, Erkasap3 Nilufer, Tosun4 Murat, Koken5 Tulay, Ozkurt3 Mete, Erkasap6 Serdar
1Dumlupinar University, Medical Faculty, Physiology Department, Kutahya, Turkey
2Dumlupinar University, Medical Faculty, Urology Department, Kutahya, Turkey
3Osmangazi University, Medical Faculty, Physiology Department, Eskiehir, Turkey
4Afyon -Kocatepe University, Medical Faculty, Histology-Embriology Department, Afyon, Turkey
5Afyon-Kocatepe University, Medical Faculty, Biochemistry Department, Afyon, Turkey
6Osmangazi University, Medical Faculty, General Surgery Department, Eskiehir, Turkey
Objective:
The aim of the present study was to evaluate the effects of phosphodiesterase type 5 (PDE5) inhibitory drugs, Tadalafil and Sildenafil, on iNOS gen expression and apoptosis in ischemia/reperfusion (I/R) induced oxidative injury in rat renal tissue.
Methods:
Eighty Sprague-Dawley rats (200–250 g) were divided into four groups. 1) control, 2) ischemia/reperfusion (I/R), 3) Tadalafil+I/R (1 mg/kg oral), and 4) Sildenafil+I/R (1 mg/kg oral) group. Rats were subjected to renal ischemia by clamping the left pedicle for 60 min, and then reperfused for 90 min. The rats were pretreated with Tadalafil in group 3 and Sildenafil in group 4, 1 h before the induction of ischemia. Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were determined in renal tissue homogenates by high-performance liquid chromatography (HPLC), and apoptotic cell assesment using TUNEL. The mRNA level of iNOS in renal tissue was determined by Real-time PCR (RT-PCR).
Results:
Our results indicate that MDA and MPO levels were increased in the I/R group than those in the control group. Both Tadalafil and Sildenafil treatment decreased the MDA levels in I/R groups, whereas this effect was more potent with Sildenafil. RT-PCR results showed that, iNOS gen expression increased in the I/R group but decreased in the PDE5 inhibitory drugs treated group. Apoptotic cells also were decreased in PDE5 inhibitory drugs-treated group.
Conclusions:
Our results indicate some beneficial effects of PDE5 inhibitory drugs on oxidative I/R injury in rat renal tissue.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC290