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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
ANTIOXIDATIVE ACTIVITY OF DRIED APRICOT DETERMINED IN VITRO BY INDUCING LIVER LIPID PEROXIDATION
Abstract number: PC275
Erden1 Yavuz, Bircan1 Burak, Eraslan2 Ersen, Tekin3 Suat
1Firat University, Faculty of Sciences, Department of Biology, Elazig
2Firat University, Faculty of Medicine, Department of Physiology, Elazig
3Inonu University, Faculty of Medicine, Department of Physiology, Malatya
Objective:
In this study, it was detected that the investigation of antioxidant capacity of apricot fruit (Prunus armeniaca) dried in the different ways on the liver lipid peroxidation (LPO) are induced with Fe2CI (Fenton's reagent; FR) in in vitro condition.
Methods:
Dry apricot samples (SO2+shine; SG, sun; G) were extracted in methanol. Two adult albino male rats were killed after anaesthetizing with mild chloroform and liver tissues were taken. The tissue samples were homogenized with Tris-EDTA buffer. After the homogenate was centrifuged, supernatant (ST) was used for in the experimental analysis. The groups were prepared as control, FR, FR+SG and FR+G. FR groups were added in ST Fe2CI solutions with fruit extracts as simultaneously and incubated for 24h. The levels of LPO was analysed on the HPLC device, according to the methods of Ohkawa et al. The total protein amount was determined by Lowry method.
Results:
The LPO amounts were increased at significant levels in all groups compared to control (p<0.0001). LPO level was decreased significantly in the FR group than in the extract (p<0.0001). The protein amount showed a partial reduction in the FR group than the control group (p<0.05), but quite a high level of total protein extract was found (p<0.0001).
Conclusions:
In conclusion, apricot samples prevented LPO. Furthermore, it showed an effect to protect proteins. It is thought that the reason for the occurrence of this effect is related with vitamins and phytochemical compounds that are contained in the apricot fruit.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC275