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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
THE PROTECTIVE EFFECTS OF DEXMEDETOMIDINE ON HEPATIC ISCHEMIA REPERFUSION INJURY
Abstract number: PC260
Kucuk1 Aysegul, Yaylak2 Faik, Cavunt Bayraktar3 Asl[inodot]han, Tosun4 Murat, Arslan5 Mustafa, Comu6 Faruk, Kavutcu3 Mustafa
1Dumlupinar University, Medical Faculty, Physiology Department, Kutahya, Turkey
2Dumlupinar University, Medical Faculty, General Surgery Department, Kutahya, Turkey
3Gazi University, Medical Faculty, Biochemistry Department, Ankara, Turkey
4Afyon-Kocatepe University, Medical Faculty, Histology-Embryogy Department, Afyon, Turkey
5Gazi University, Medical Faculty, Anesthesiology and Reamination Department, Ankara, Turkey
6Krkkale University, Medical Faculty, Physiology Department, Krkkale, Turkey
Objective:
The aim of this study was to evaluate the effect of dexmedetomidine (100mg/kg-i.p.) on liver ischemia and reperfusion (I/R) in rats.
Methods:
Twenty-four Wistar Albino rats were separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 minutes and reperfusion period was 45 minutes after declampage. Group I/R-D received dexmedetomidine 100mg/kg i.p. 30 min before portal clampage. Malondialdehyde (MDA), glutathione-S-transferase (GST), superoxide dismutase (SOD), Catalase (CAT), and paraoxonase 1 (PON-1) were investigated. Also HSP60 and p53 positive hepatocytes were counted under UTHSCSA Image Tool for Windows 3.0 image analysis program.
Results:
All parameters, except GST levels, were significant between the groups (p<0.05). Although HSP60 expression was significantly increased between I/R, I/R-D and C group p=0.015 and 0.001, respectively), there were no significant differences between I/R-D and C (p=0.443). On the other hand, p53 expression was also significantly increased between I/R, I/R-D and C group (p=0.044 and 0.003 respectively). At the same time, there were no significant differences between I/R-D and C group (p=0.354).
Conclusions:
All the results suggest that Dexmedetomidine has beneficial effects on liver ischemia/reperfusion stress.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC260