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Acta Physiologica Congress

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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey


ASSOCIATION OF LEPTIN GENE -2548 G/A POLYMORPHISM WITH PLASMA LEVELS IN TURKISH PATIENTS WITH OBESITY
Abstract number: PC172

Say Sahin1 Deniz, Tmer1 Cemil, Demir2 Cemil, Ucar3 Edip, Murat Celik3 Muhammet, Gunesacar4 Ramazan

1Mustafa Kemal University Faculty of Medicine Department of Physiology
2Mustafa Kemal University Faculty of Medicine Department of Central Laboratory
3Mustafa Kemal University Faculty of Medicine Department of Internal Medicine
4Mustafa Kemal University Faculty of Medicine Department of Medical Biology and Genetics

Objective: 

Leptin is a product of the obesity gene which is secreted by mostly adipose tissue, operates by inhibiting food intake and stimulating energy expenditure. The gene encoding leptin (LEP) is located in human beings on long arm of chromosome 7 at band q31.3 and is organized into three exons that are separated by two introns. Leptin may play an important role in the pathogenesis of obesity. In the present study, we aimed to investigate the association of LEP -2548 G/A polymorphism with plasma leptin levels in Turkish obese patients.

Methods: 

Sixty obese patients and 60 healthy controls were included in this study. Plasma leptin levels were determined using ELISA technique. Leptin gene promoter -2548G/A genotype was determined by polymerase chain reaction followed by a digestion with the Hha I restriction enzyme.

Results: 

Plasma leptin levels were significantly higher in obese patients (p<0.0001). In the obese patients, AA and GA genotype frequencies were higher than in healthy controls (p<0.016 for AA genotype; p<0.011 for GA genotype). A allele frequency was also found to be higher in obeses than those of healthy subjects (p=0.0001). In the entire study sample, LEP -2548 A allele carriers (AA+GA) had significantly higher levels of plasma leptin than subjects with GG genotype (p<0.0001). Obese patients carrying the A allele exhibited higher leptin level than homozygous for G allele (p<0.0001).

Conclusions: 

Present results showed that LEP -2548 GA genotype or A allele is associated with higher plasma leptin levels and this variant may contribute to the development of obesity in Turkish population.

To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC172

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