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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
THE EFFECTS OF DOCOSAHEXAENOIC ACID (DHA) ON INOS AND BCL-2 EXPRESSION LEVELS IN BILATERALLY MPTP-LESIONED RAT MODEL OF PARKINSONS DISEASE
Abstract number: PC139
Hacioglu1 Gulay, Seval Celik2 Yasemin, Tanriover2 Gamze, Ozsoy1 Ozlem, Saka Topcuoglu3 Esen, Balkan4 Sevin, Agar1 Aysel
1Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
2Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
3Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
4Department of Neurology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Objective:
The aim of this study was to observe whether the neuroprotective effects of DHA in an experimental Parkinson model is related with alterations in iNOS and Bcl-2 expressions.
Methods:
Adult male Wistar rats were divided into 4 groups: Control; DHA-treated; MPTP-induced; MPTP-induced+DHA-treated. Motor activity was investigated by vertical pole test. The lesion was determined by immunohistochemical analysis for tyrosine hydroxylase (TH)-immunopositive cells in substantia nigra (SN). Immunoreactivities of iNOS and Bcl-2 in SN were evaluated by immunohistochemistry.
Results:
MPTP-induced animals exhibited decreased locomotor activity and motor coordination. Diminished symptoms and decreased dopaminergic neuron death were detected in the MPTP-induced+DHA-treated group compared to MPTP-induced group. iNOS immunoreactivity increased in MPTP-induced group compared to control and DHA, however it was decreased in the MPTP-induced+DHA-treated group compared to the MPTP-induced group. The staining intensity for Bcl-2 decreased in the MPTP-induced group compared to control, while it was stronger in the MPTP-induced+DHA-treated group compared to MPTP-induced group.
Conclusions:
The alterations in the expression levels of iNOS and Bcl-2 would be possible factors in neuroprotective effects of DHA in experimental model of Parkinson's disease.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC139