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Acta Physiologica Congress

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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey


THE EFFECT OF ESTROGEN ON ETHANOL INDUCED BLOOD BRAIN BARRIER DISRUPTION IN AN IN VITRO MODEL
Abstract number: PC123

Karadenizli1 Sabriye, Uzm1 Gülay, Erdem Kuruca1 Serap, Ak Gun Dar2 Kadriye, Cetin1 Beyza, Kapucu2 Ay[scedil]egul

1Department of Physiology, Medical faculty of istanbul,Istanbul University, Istanbul, Turkey
2Department of Biology, Facultyof Science, Istanbul University, Istanbul, Turkey

Objective: 

The blood-brain barrier (BBB) is formed by the presence of tight junction proteins (TJPs)between brain endothelial cells that restrict paracellular permeability and it is essential for central nervous system normal function. The effect of estrogen on BBB function is unclear. It is reported that age and estrogen reseptors are important for its efects. We performed this study to determine 17beta-estradiol could affect blood-brain barrier disruption caused by ethanol using an in vitro BBB model.

Methods: 

Measurements of transendothelial electrical resistance (TEER) and expression of TJPs (occludin and claudin 1-2) were performed to analyze BBB integrity and inducibility in an in vitro co-culture model of human umbilical vascular endothelial cells (HUVEC) and rat glioma cells (C6). HUVEC express estrogen receptor beta but not alfa (It is intresting in terms of imitating menopause period).

Results: 

Coculture of HUVEC/C6 caused increase in TEER and TJPs levels indicating BBB formation. Adding ethanol into the culture media after BBB formation, BBB integrity was destroyed (decrease in TEER and TJPs). Effect of 17 beta estrodiol on BBB: long-term application of estrodiol, its single application, its application before and after ethanol caused decrease both in TEER and in the amount of TJPs. This decrease was higher when ethanol and estrogen were applied together.

Conclusions: 

Preventing the formation of the BBB in HUVEC line and increasing of ethanol induced disruption of barrier with addition of estradiol, let us think that estrogen treatment (especially in menopause) can create a risk for causing cerebrovascular diseases.

To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC123

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