Objective: 

Oxidative stress is suggested to have an important role in the development of neurological complications in diabetes. Some studies have demonstrated that medical ozone treatment reduced reactive oxygen species by stimulation and/or preservation of the endogenous antioxidant systems in experimental models. The aim of this study was to investigate the protective effects of medical ozone treatment on learning- memory functions in diabetic rats.

Methods: 

Thirty adult female Sprague Dawley rats were divided into five groups (6 rats in each); Control group (C), Diabetes group (D), Ozone group (O), Diabetes+Ozone group (DO), Diabetes+Insulin group (DI). For induction of experimental DM, Streptozocine was injected intra peritoneally. Ozone and insulin injections in diabetic rats were given to intraperitoneally. After fifteen days, seven day Morris Water Maze protocol was used to evaluate for learning process. Kruskal-Wallis and Mann-Whitney U tests were used for the differences between groups, P<0.05 accepted as statistically significant.

Results: 

Memory retrieval was increased DO and DI groups compare to D groups but this increase was not statistically significant.

Conclusions: 

A longer period of medical ozone treatment may prevent memory retrieval impairment induced by diabetes mellitus.