Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
ANTINEOPLASTIC PROPERTIES OF MONENSIC ACID AND ITS BIOMETAL (II) COMPLEXES
Abstract number: PC027
Alexandrova1 Radostina, Zhivkova1 Tania, Ivanov1 Dimitar, Alexandrov1 Marin, Kirilova2 Milena, Miloshev2 George, Pantcheva3 Ivayla, Mitewa3 Mariana
1Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria
2Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria
3Faculty of Chemistry, Sofia University St. Kliment Ohridski, Sofia, Bulgaria
The polyether ionophore Monensin is a natural antibiotic, applied in veterinary medicine as coccidiostatic and antibacterial agent. Recent data have revealed that Monensin expresses also antitumor activity against cell lines from various malignancies including leukemia, lymphoma, myeloma, renal cell carcinoma and cancers of the colon, breast and cervix. The aim of our study was to evaluate the putative antineoplastic properties of metal complexes of Monensic acid (MonH) - [M(Mon)2(H2O)2] (M = Mg, Ca, Mn, Co, Ni, Zn).
The following model systems were used in our experiments: cultured human permanent cell lines established from glioblastoma multiforme (8MGBA) and cancers of the lung (A549), breast (MCF-7), uterine cervix (HeLa), liver (HepG2) and skin (A431); virus-transformed chicken and rat tumor cells expressing v-myc or v-src oncogene, respectively; primary cultures of chemically induced liver cancer of Zajdela in rat. The investigations were carried out by MTT test, neutral red uptake cytotoxicity assay, crystal violet staining, trypan blue dye exclusion technique, method of Bradford and colony forming method. The ability of the compounds to induce double stranded DNA damages was examined by Comet assay. Double staining with acridine orange and propidium iodide was applied to demonstrate the presence of cytopathological changes.
The results obtained reveal that applied at concentrations of 0.525 microg/mL for 2472 h the examined compounds decreased significantly the viability and proliferation of the treated cells in a time- and concentration-dependent manner.
These metal(II) complexes have been found to exhibit higher cytotoxic and cytostatic activities as compared to the non-coordinated Monensic acid. Acknowledgement: Supported by Grant DO-02-84/2008, National Science Fund, Bulgaria.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC027