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Acta Physiologica Congress

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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey


COMPARISON OF MELATONIN AND OZONE IN THE PREVENTION OF REPERFUSION INJURY FOLLOWING UNILATERAL TESTICULAR TORSION: AN EXPERIMENTAL STUDY IN RATS
Abstract number: OC06

Ekici1 Sinan, Ekici2 I[scedil][inodot]n Do[gbreve]an, Ozturk3 Güler, Aksungar4 Fehime, Turan1 Güven, Luleci5 Nurettin

1Department of Urology, Maltepe University, Istanbul, Turkey
2Department of Pathology, Yeditepe University, Istanbul, Turkey
3Department of Physiology, Maltepe University, Istanbul, Turkey
4Department of Biochemistry, Maltepe University, Istanbul, Turkey
5Department of Algology, Maltepe University, Istanbul, Turkey

Objective: 

Testicular torsion/detorsion (TdT) is an ischemia/reperfusion injury (IRI). Melatonin has been shown as the most powerful antioxidant in attenuation of testicular IRI. We investigated the efficacy of ozone in comparison with melatonin in an experimental rat model of TdT.

Methods: 

Twenty four male Wistar rats were divided into four groups designed as sham-operated, TdT, TdT plus melatonin and TdT plus ozone. The intraperitoneal injected doses of melatonin and ozone were 10 mg/kg/day and 4 mg/kg/day, respectively. Melatonin and ozone were injected daily beginning 15 minutes before detorsion for the following 7 days. At seventh day, blood and tissue samples were obtained. Johnsen score (JS), malondialdehyde (MDA), inhibin B (IB), reduced glutathione (GSH) plasma total sulfhydryl group (RSH) levels and nitric oxide (NOx) were studied. This study was approved by the local ethics committee.

Results: 

TdT caused increases in tissue MDA and NOx along with decreases in JS, tissue and plasma IB and GSH levels. Melatonin prevented the rise in MDA and NOx levels and improved JS, tissue and plasma IB and tissue GSH levels along with decreases in plasma RSH level. Ozone presented similar results except for NOx level. Concomitantly, in contralateral testis, similar but insignificant changes were seen in JS, MDA and IB by melatonin and ozone, along with significant changes in GSH level. Melatonin caused a decrease, but ozone caused an increase in NOx level in both testes.

Conclusions: 

On different pathways, ozone was comparable with melatonin in amelioration of IRI. Protective effects of ozone were associated with NO. The potential for ozone as a treatment for TdT, therefore, deserves to be further elucidated.

To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :OC06

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