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Acta Physiologica Congress

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Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey


CELL SIGNALING AND CYTOKINE SECRETION RESPONSE DIFFERENCES IN MIDKINE SILENCED AND OVEREXPRESSED RAT ALVEOLAR MACROPHAGES
Abstract number: W15

Yazihan1 Nuray, Sahin2 Fikret, Ermis3 Ezgi, Yilmaz3 Evin, Kacar Kocak4 Mehtap, Akcil1 Ethem, Baloglu5 Emel, Mairbeurl5 Heimo

1Ankara University, Faculty of Medicine, Pathophysiology Department, Ankara, Turkey
2Ankara University, Faculty of Medicine, Microbiology Department, Ankara, Turkey
3Ankara University, Faculty of Medicine, Molecular Biology R&D Unit Ankara, Turkey
4Yeditepe University, Faculty of Medicine, Pathophysiology Department Istanbul, Turkey
5Heidelberg University, Sport Medicine Division, Heidelberg, Germany

Hypoxia, which commonly associates with respiratory and cardiovascular diseases, provokes an acute inflammatory response. Molecular mechanisms of the inflammatory reaction in hypoxia-induced lung injury are not well defined. Evidence obtained in intact animals and in primary cell cultures indicate that alveolar macrophages activated by hypoxia release a mediator(s) into the circulation. Midkine is one of the molecules playing a significant role in the control of inflammatory processes. It is a heparin-binding growth factor which promotes cell adhesion, migration, proliferation and survival. In this study, we aimed to evaluate effect of midkine in cytokine secretion responses of alveolar macrophages in normoxia and hypoxia. Midkine expression in rat alveolar macrophage cell line NR8383 was silenced using midkine siRNA sequence primers and overexpressed. Quantitative RT-PCR assay was performed to quantify the mRNA expression changes of midkine. Cells were exposed to K pneumonia lipopolysaccaride (LPS) in normoxic and hypoxic conditions. TNF-a, IL-4, IL-5, IL-6, IL-10 levels were measured by ELISA. Cell proliferation rate was higher in midkine overexpressed cells. Basal cytokine secretion levels and cytokine response to LPS increased in midkine overexpressed cells whereas it was decreased in midkine silenced cells. Midkine overexpressed cells showed increased NFkb, p38/MAPK, JNK, protein kinase C and erk activation compared to control and silenced cells. Our results showed that midkine is important for cytokine secretion, intracellular signalling functions and survival of alveolar macrophage cells.

This study was supported by TUBITAK-BMBF (SBAG108S262).

To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :W15

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