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Acta Physiologica 2011; Volume 202, Supplement 684
The Joint Conference (FAMÉ 2011) of the LXXVth Meeting of the Hungarian Physiological Society, XVIth Meeting of the Hungarian Society of Anatomists, Experimental Section of the Hungarian Society for Experimental and Clinical Pharmacology and Hungarian Society for Microcirculation and Vascular Biology
6/8/2011-6/11/2011
Pécs, Hungary
EFFECT OF KIDNEY ISCHEMIC POSTCONDITIONING AND PHARMACOLOGICAL POST-TREATMENT ON IN HEALTHY AND DIABETIC RATS
Abstract number: O34
Miklos1 Zs., Kurthy1 M., Lantos1 J., Vida2 M., Weber1 Gy., Jancso2 G., Roth1 E.
Aims:
Ischaemic and pharmacological postconditioning (PC) protect against ischaemia-reperfusion (IR) injury. Metabolic alterations, such as diabetes mellitus reduce or abolish the beneficial effects of these maneuvers. Big Endothelin is the biological precursor of Endothelin, the most potent vasoconstrictor known today. The aim of our work was to investigate the effect of ischaemic postconditioning, post-treatment by insulin and wortmannin after kidney IR by measuring the plasma big Endothelin level in healthy and diabetic rats.
Methods:
Healthy and streptozotocin induced diabetic male albino rats of the Wistar strain (300–400 g) were used in the study. Anaesthetized rats underwent to both kidney pedicle cross-clamping for 45 min. Ischaemia was followed by 120-min reperfusion with or without postconditioning protocol. Postconditioning was induced by four intermittent periods of ischaemia-reperfusion of 15 sec duration each. Rats were randomly divided into four groups: rats underwent to IR (group I), IR+PC (group II); IR and insulin treatment 5 min before reperfusion (group III); IR+PC and phosphatidylinositol-3-kinase inhibitor wortmannin treatment before reperfusion (group IV). Urine urea, creatinine and serum big Endothelin levels (big Endothelin ELISA kit, Biomedica) were measured before and after surgery.
Results:
No significant differences were detected in urine urea and creatinine levels in experimental groups. Big Endothelin level was significantly higher in diabetic rats compared to healthy ones (1.027±0.198 vs. 0.841±0.193 fmol/ml, p<0.01). Postconditioning with wortmannin caused a significant reduction in serum big Endothelin levels (0.953±0.113 vs. 0.722±0.138 fmol/ml) in healthy animals.
Conclusion:
Diabetes induced significant big Endothelin elevation in rats. Ischaemic postconditioning and wortmannin treatment significantly decreased the big Endothelin level in healthy animals. Ischaemic postconditioning and insulin treatment did not affect the level of big Endothelin neither in the healthy nor in the diabetic rats.
Support:
OTKA- K67731; AOKKA-34039-28/2009; OTKA: K78434
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 202, Supplement 684 :O34