Back
Acta Physiologica 2011; Volume 202, Supplement 684
The Joint Conference (FAMÉ 2011) of the LXXVth Meeting of the Hungarian Physiological Society, XVIth Meeting of the Hungarian Society of Anatomists, Experimental Section of the Hungarian Society for Experimental and Clinical Pharmacology and Hungarian Society for Microcirculation and Vascular Biology
6/8/2011-6/11/2011
Pécs, Hungary
INVESTIGATION OF THE ROLE OF WATER-SOLUBLE CINNAMOMUM EXTRACT IN THE PREVENTION OF ATHEROSCLEROSIS
Abstract number: P54
Lepran1 Á., Torok1 D., Mester1 G., Mecs1 Z., Szabo1 Gy., Redei3 D., Hohmann3 J., Lepran2 I., Mezei1 Zs.
Aims:
The extract of Cinnamomum cassia bark produces antidiabetic and antioxidant effects. Cinnamaldehyde, the main constituent of its volatile oil, reduces platelet activation. Activated platelets and reactive oxygen metabolites play a basic role in the pathogenesis of atherosclerosis. The progress of atherosclerosis could be slowed down by cinnamon, however, some of its lipophilic components may have allergic and toxic effect. The aim of our experiments was to investigate the effects of a water-soluble extract of Cinnamomum cassia bark on platelet aggregation, vascular contractility and eicosanoid synthesis in Wistar-Kyoto rat.
Methods:
The composition of water-soluble extract of cinnamomum was measured by HPLC. We examined the effect of the extract (25–400mg/ml) on the eicosanoid synthesis of platelets and aorta (applying 1-14C-arachidonic acid, as a substrate and RIA), the contractility of isolated aorta ring (KCl 80mM, phenylephrine/Phe 1mM), and on platelet aggregation (ADP 10mM).
Results:
The cinnamomum-aldehyde content of our water extract was 0,177±0,015%. The formation of platelet cyclooxygenase metabolites (COX) was reduced with increasing concentrations of the extract. The vasoconstrictor and platelet aggregator thromoboxane A2 synthesis was already decreased by 50mg/ml extract (16673±1016 vs. 13054±1154 dpm). Cinnamomum extract (300mg/ml) significantly reduced the primary (37%) and secondary (97%) aggregation of platelets. The total amount of COX metabolites (57%) and the synthesis of 6-keto-PGF1a (40%) in aorta was increased by 25mg/ml water-soluble extract, while both inhibited by 400mg/ml extract. The water-soluble cinnamomun extract (25mg/ml) induced endothelial dependent relaxation (55%) of Phe pre-contracted aorta rings. Both KCl (20%) and Phe (23%) induced contractions of aorta rings were reduced by cinnamomun pretreatment.
Conclusions:
These results may suggest that a water-soluble cinnamomum extract – by reducing the sensitivity of platelets to activators and increasing the protective role of endothelial cells – could slow down the progress of atherosclerosis.
Support:
ETT355-08/2009, TÁMOP 4.2.2.-08/1-2008-0013; 4.2.1./B-09/KONV-2010-005
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 202, Supplement 684 :P54