Back
Acta Physiologica 2011; Volume 202, Supplement 684
The Joint Conference (FAMÉ 2011) of the LXXVth Meeting of the Hungarian Physiological Society, XVIth Meeting of the Hungarian Society of Anatomists, Experimental Section of the Hungarian Society for Experimental and Clinical Pharmacology and Hungarian Society for Microcirculation and Vascular Biology
6/8/2011-6/11/2011
Pécs, Hungary
TREATMENT POSSIBILITIES TO RESTORE INTESTINAL PERFUSION IN A PORCINE MODEL OF CARDIAC TAMPONADE
Abstract number: O21
Kaszaki1 J., Erces1 D., Okada2 H., Zimmermann3 T., Ghyczy1 M., Boros1 M.
Aims:
Cardiac tamponade (CT) is a severe clinical syndrome most often caused by high-energy thoracic injuries. Due to the fast circulatory redistribution, CT leads to unmanageable peripheral microcirculatory complications, gastrointestinal hypoperfusion and mucosal barrier failure with bacterial translocation. These events are linked to inflammatory activation with increased production of reactive oxygen- and nitrogen intermediates. Our aim was to investigate the comparative anti-inflammatory effects of complement C5a antagonist (C5AA; Anabloc) treatment, methane inhalation (which proved to be effective radical scavenger) and selenium (Se), a known cofactor of enzymes of the redox balance, on the hemodynamics and microcirculatory changes in a large animal model of CT.
Methods:
In anaesthetized, ventilated and thoracotomized minipigs CT was induced for 60 min by intrapericardial administration of colloid solution, meanwhile the mean arterial pressure was kept between 40–45 mmHg. Macrohemodynamic changes, small intestinal pCO2 gap values (by tonometric probe), blood superoxide production (chemiluminometric method) were monitored for 180 min. The CT+C5AA group was treated with 4 mg/kg Anabloc at the 45th min of tamponade. The methane-treated group (CT+Met) was ventilated with 2.5% methane/air mixture, starting at the end of CT, and lasting for 20 min. The CT+Se group was treated with continuous Se infusion (25 mg/kg/h iv) after CT induction. The fifth group served as sham-operated control.
Results:
After CT cardiac output was normalized, but the increase in the intestinal pCO2 gap (34±8 vs. 53±9 mmHg) and the superoxide production of blood referred to the peripheral microcirculatory impairment. All treatments significantly decreased the superoxide production and improved the mucosal microcirculation (CT+C5AA 29±7; CT+Met: 26±6 CT+Se 36±7 vs. CT 53±9 mmHg).
Conclusion:
The different treatments had nearly equal effectiveness, thus reactive oxygen radicals, produced by different ways, are important common denominators of CT-induced gastrointestinal microcirculatory dysfunction.
Support:
ETT 442/2009, OTKA K75161; TÁMOP4.2.1; TÁMOP-4.2.2; TÉT JP 16/09
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 202, Supplement 684 :O21