Back
Acta Physiologica 2011; Volume 202, Supplement 684
The Joint Conference (FAMÉ 2011) of the LXXVth Meeting of the Hungarian Physiological Society, XVIth Meeting of the Hungarian Society of Anatomists, Experimental Section of the Hungarian Society for Experimental and Clinical Pharmacology and Hungarian Society for Microcirculation and Vascular Biology
6/8/2011-6/11/2011
Pécs, Hungary
THE EFFECTS OF REMOTE LIMB ISCHEMIC PRECONDITIONING ON THE CONSEQUENCES OF PARTIAL HEPATIC ISCHEMIA IN RATS
Abstract number: P27
Hartmann1 P., Garab1 D., Boros1 M., Szabo1 A.
Aims:
We set out to investigate how a remote ischemic protocol can influence the biochemical and microcirculatory consequences of ischemia-reperfusion injury (I/R).
Methods:
In a rodent model of partial hepatic ischemia (male Sprague-Dawley rats; 250g BW), the left lateral branches of the portal vessels were occluded for 60 min (n=8). Partial hepatic warm ischemia was followed by reperfusion in the presence or absence of preceding limb ischemic preconditioning (IPC, 2 x 10 min ischemia/reperfusion cycles, n=7). On-line visualization of the leukocyte-endothelial interactions was performed with fluorescence intravital videomicroscopy; tissue perfusion and oxygen saturation were simultaneously measured with the O2C ("O2-to-see") technique. Serum levels of AST, ALT, LDH and hepatic xanthine oxidoreductase (XOR) and myeloperoxidase (MPO) enzyme activities from tissue homogenates were also assessed. The data we compared with sham-operated animals (n=5).
Results:
I/R caused an approx. 4-fold increase in the number of rolling, and a 3-fold increase in the number of adherent PMNs in the central postsinusoidal venules of the affected liver lobe as compared to the values of sham-operated animals (rolling: 64±12 1/mm/s; sticking: 61±7 1/mm2). In parallel, blood flow and tissue oxygen saturation were decreased with 20%, respectively (from ~230 AU and ~46% control values). These changes were associated with a 2-fold increase MPO and a 4-fold elevation in XOR activity as compared with the sham-operated animals. Remote IPC elicited on the limb resulted in significant improvements in the microcirculatory parameters (perfusion, oxygen saturation, cell-to-cell interactions), ameliorated hepatic oxidative stress and leukocyte infiltration and moderately improved liver enzymes.
Conclusions:
The results show that ischemic limb preconditioning beneficially influences the postischemic microcirculatory reactions induced by liver I/R. Remote preconditioning of an extremity can easily be performed in the clinical practice, and it is a potential tool to ameliorate the microcirculatory failure of a distant organ.
Support:
TAMOP-4.2.1/B-09/1/KONV-2010-0005 and TAMOP-4.2.2-08/1-2008-0013
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 202, Supplement 684 :P27