Tumor cells utilize preferably glucose for energy production. They accomplish cellular glucose uptake in part through Na⁺-coupled glucose transport mediated by SGLT1 (SLC5A1). Little is known about mechanisms upregulating SGLT1 in tumor cells. The present study explored the possibility that the human papilloma virus protein HPV-18E6 participates in the stimulation of SGLT1 activity. According to Western blotting, SGLT1 is expressed in HELA cells. To explore whether HPV-18E6 affects SGLT1 activity, SGLT1 was expressed in Xenopus oocytes with and without HPV-18E6 and electrogenic glucose transport determined by dual electrode voltage clamp. In SGLT1-expressing oocytes but not in oocytes injected with water or expressing HPV-18E6 alone, the addition of glucose to the extracellular bath generated a current (I_g). I_g was significantly increased by coexpression of HPV-18E6. According to chemiluminescence and confocal microscopy, coexpression of HPV-18E6 significantly increased the SGLT1 protein abundance in the cell membrane. The decay of I_g following inhibition of the carrier insertion by brefeldin (5 mM) treatment was not significantly affected by coexpression of HPV-18E6, indicating that HPV-18E6 was rather effective by stimulating the carrier insertion into the cell membrane. In conclusion, HPV-18E6 participates in the upregulation of SGLT1 activity in tumor cells.