Objective: 

To observe the real-time influence of Xuesaitong injection on the collagen I induced platelet adhesion and aggregation.

Methods: 

Bioflux1000 microfluidic device was employed in this study for the first time. The observation channels were coated with collagen I. SD rats underwent anesthesia and blood withdrawn from aortic vessels with 3.8% sodium citrate 1:9 anticoagulation. Whole blood with Calcein AM (4mM) fluorescent labeling for 1 hr. Different concentration of Xuesaitong Injection were added to whole blood incubated for 10 minutes in room tempreture. Whole blood was perfused from the wells at different shear stress for 5 minutes. Fluorescent micrographs were captured with the blood under flow using an inverted microscope and CCD Camera. Inhibition rates by medicine were calculated by percent of control.

Results: 

Exposure to collagen I mediated stable platelet adhesion and aggregation within two to three minutes under 10dyn/cm² flow. The IC₅₀ of Xuesaitong injection on inhibiting platelet adhesion and aggregation is 0.25mg/ml under 10dyn/cm² flow. By curve estimate we found the logarithm relation between shear stress and efficacy (R²=0.97).

Conclusion: 

Xuesaitong injection could inhibit collagen-induced platelet adhesion and aggregation under flow; Shear stress showed impact on the efficacy of medicine; Bioflux1000 could be used to evaluate the efficacy of Chinese medicine.

Figure 1