Akt2 is an uibiquitous kinase downstream of phosphoinositide-3-kinase (PI3K) and phosphoinositide dependent kinase (PDK1), which are involved in signal transduction of psychotropic drugs and brain derived growth factor (BDNF) signalling. BDNF influences neuronal plasticity, mood, long lasting memory formation and is implicated in multiple psychiatric disorders. PI3K-Akt signaling is further implicated in the beneficial effects of insulin and erythropoietin on mood disorders and/or schizophrenia. In order to explore the in vivo functional significance of Akt2 in the regulation of exploratory behavior and anxiety related responses, we assessed the performance of Akt2 knockout mice (akt2^-/-) and corresponding Wildtype controls during behavioral testing. As a result, akt2^-/- mice observed in light/darkbox experiments showed no difference in the exploratory behaviour measured by visits in the light region, yet spent significantly more time in the protected dark area and took longer to leave the entrance area. While there was no difference between akt2^-/- and WT controls in walking distance, akt2^-/- mice spent significantly more time in corners and showed increased centre field avoidance in the open-field experiment. Also, rearings per time were significantly decreased in the akt2^-/- mice. In conclusion, lack of Akt2 leads to subtle behavioral defects. The anxiety-prone behaviour of the akt2^-/- mice indicates a distinct role of the Akt2 in the regulation of neuronal processes.