Physical stimulation of body surface points is known to affect various organ functions. In traditional Chinese medicine so called acupoints were defined. These points can be physically stimulated to effectively treat various diseases. Here the effect of CO₂-laser-stimulation at the acupoints Neiguan (PC-6), Quchi (LI-11), Zusanli (ST-36) and Taichong (LR-3) on heart rate and mean arterial blood pressure was investigated in anesthetized rats. Our results revealed that the laser stimulation at left or right PC-6 and LR-3 increased heart rate and mean arterial pressure. There was no response of heart rate and mean arterial pressure during and after stimulation of the left LI-11, but laser stimulation at the right LI-11 also increased heart rate and mean arterial pressure. On the other hand, laser stimulation at left and right ST-36 decreased heart rate and mean arterial pressure. The effects on mean arterial pressure were more pronounced than those on heart rate. After full spinal cord transection all heart-rate and mean-arterial-pressure responses were attenuated or completely abolished. These results suggest that CO₂-laser stimulation at either left or right PC-6, ST-36 and LR-3, as well as at the right LI-11 can modulate the cardiovascular functions in anesthetized rats, and its modulatory site might be supraspinal.