Premenopausal women show different blood pressure control compared to men. Sex hormones may be responsible for this phenomenon. Kidney perfusion and filtration have been related to blood pressure control. We therefore test the hypothesis that sex hormone such testosterone and estradiol influence vasoreactivity of interlobar arteries from mice. Interlobar arteries were obtained from C57BL6 female and male mice and mounted in wire myograph under isometric conditions. Non-genomic effects of 17-beta-estradiol and testosterone were investigated. Responses to phenylephrine were similar in vessels of female and male mice. However, female mice showed negligible reaction to Ang II, while vessels of male mice contracted up to 12% of the maximum KCl response. Female vessels dilated stronger to acetylcholine compared to male. Testosterone and estradiol relaxed pre-constricted vessels dose dependently and similarly. There were no sex related differences for this dilator action. Pretreatment with testosterone and estradiol only slightly modulated the concentration response curves for phenylephrine, angiotensin II and acetylcholine in female and male mice. The study demonstrates sex differences of the Ang II action in renal arteries, which cannot be related to a differential, non-genomic, dilator effect of estrogens and testosterone, since both hormones induced similar dilatation. Further investigations are necessary for identifying the underlying mechanisms of these sex related differences in renal artery function.