Background: 

The polyphenols derived from red wine, as the catechin, possess highly antihypertensive properties so the possibility of their use in vitro is studied since they prevent oxidative stress-induced endothelial dysfunction. The aim of this work is to examine the role of the reactive oxygen and nitric oxide species (ROSs/RNSs) in the damage induced by hypothermia in HUVECs considering also the interaction with Ang II, Losartan, PD123319.

Materials and Methods: 

Authors analyzed the effect of the Cathechin (50 mM) and of the Angiotensin II, Losartan, PD123319 both in normal and in injured cells at long times of culture. After thermal damage at temperatures of 33–34° the cells were incubated with different dyes: Acridine Orange for vitality test, 2¹7¹-diclorodiidrofluoresceina diacetate for the evaluation of ROS production, the DAF-2DA for RNSs and Propidium Iodide for tested molecular damage on DNA of Huvecs. The images were captured by Cell-A program on Olympus IX 50 microscope and intensity of fluorescence was evaluated by NIH Image J program.

Results: 

The data obtained show after hypotermic shock: proliferation increase in samples treated with Ang II and PD123319; decreases the production of ROS for the samples treated with Ang II, Losartan and PD123319 and Catechins; decreases of NO production in all treated samples (Ang II and Catechin), increases of nuclear damage but not in presence of Catechins.