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Acta Physiologica Congress

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Acta Physiologica 2010; Volume 198, Supplement 677
Joint Meeting of the Scandinavian and German Physiological Societies
3/27/2010-3/30/2010
Copenhagen, Denmark


,-MEATP-MEDIATED FACILITATION OF NECK MUSCLE NOCICEPTION INVOLVES NNOS AND INOS IN ANAESTHETIZED MICE
Abstract number: P-MON-123

RISTIC1 D, ELLRICH1 J

Objectives: Intramuscular (i.m.) a,b-meATP (ATP) infusion into murine neck muscles induces sustained facilitation of brainstem nociceptive processing. Systemic (i.p.) application of unspecific nitric oxide synthase (NOS) inhibitor prevents and reverses the ATP-effect. The three isoenzymes neuronal (nNOS), inducible (iNOS) and endothelial NOS might contribute to the effect. The experimental study addressed the possible involvement of nNOS isoenzyme. Methods: Electrophysiological experiments were conducted in 45 anaesthetized mice. Infusion of 1 mM ATP (20 ml) served as noxious stimulus to semispinal neck muscles. Brainstem nociception was monitored by the jaw-opening reflex (JOR) elicited via electrical tongue stimulation. Two experiments were conducted. After JOR baseline, nNOS inhibitor NPLA (0.5, 1, 2 mg/kg, i.p.) or saline (isotonic) was administered 30 minutes before local ATP-infusion. JOR was recorded for further 90 minutes. In the second experiment, NPLA, iNOS inhibitor 1400W (2 mg/kg, i.p.) or saline was injected 90 minutes after local ATP- application under established reflex facilitation. JOR monitoring continued for at least 60 minutes. Results: Sole NPLA injection did not alter basal JOR (n.s.). Preceding application of NPLA prevented ATP-induced facilitation dose-dependently (p<0.001). ATP induced facilitation with either preceding saline (p<0.001) or 0.5 mg/kg NPLA (p<0.001). The ATP-effect was prevented with 1 and 2 mg/kg NPLA. Subsequent saline and NPLA injection did not affect established facilitation. Subsequent 1400W reversed facilitation back to baseline (p<0.001). Conclusions: nNOS is involved in the induction of purinergic facilitation of brainstem nociception. iNOS contributes to its maintenance. This points to differential roles of NOS isoenzymes in ATP- mediated facilitation of neck muscle nociception.

To cite this abstract, please use the following information:
Acta Physiologica 2010; Volume 198, Supplement 677 :P-MON-123

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