Introduction: Recent development of nanomedicine brought new research directions regarding single wall carbon nanotubes (SWCNT) biomedical applications. However, the role of oxidative stress is incompletely elucidated using in vivo studies. Material and method: High purity (97%) SWCNT were functionalized with single strand DNA (ss-DNA) through 7 hours sonication. NaCl was added up to the concentration of 0.9%. Estimated concentration of SWCNT-SS-DNA solution was 390mg/L. Wistar rats (170±10 g) were used for the experiment. Subcutaneous injection of 0.5 ml of the solution was performed to one group (test group). Control group received 0.5 ml serum through subcutaneous injection. Levels of malondialdehyde (MDA), protein carbonyls (PC), hydrogen donors (HD), sulfhydril groups (SH) were determined in plasma and liver tissue homogenate at 3, 6, 24 and 48 hours from administration. Values recorded for the two groups were compared. Results: Plasma levels of MDA (p<0.05) and CP (p<0.05) increased starting from 6 hours post-injection. Within the same interval we recorded decrease of HD and SH (p<0.05) plasma level. Decrease of MDA (p<0.05), PC (p<0.05) levels were recorded until 24 hours post-injection, followed by increased levels after this interval. Inverse alterations were recorded for HD and SH (p<0.05). Conclusions: Subcutaneous administration of ss-DNA-SWCNT in solution modifies the oxidant /antioxidant balance in plasma and liver tissue. Effects are dependant on material biodistribution.