Aims: Various purinergic P1 and P2 receptors have been identified in rat pancreatic ducts and human pancreatic duct cell lines. The aim of this study was to develop a model for studying the function of purinergic receptors in human pancreatic duct cell line, Capan-1. Methods: Capan-1 cells were grown as monolayer on permeable supports. Electrical parameters were measured in open-circuit Ussing chamber in physiological buffers with and without bicarbonate. Results: Capan-1 cells had functional secretin, VIP and cholinergic receptors. Carbachol (10^-5 M) hyperpolarized transepithelial potential, V_t, from -0.5 ±0.3 mV to -1.1 ±0.4 mV (n=7). Secretin and VIP (10^-8 M) increased V_t by about 1.5 mV. Forskolin made V_t more negative by 1.6 ±0.6 mV (0.01 mM, n=5). Purinergic agonists (ATP, UTP, Adenosine, BzATP, UDP; 0.1 mM) loaded on either side induced a transient effect of V_t. The rank order of the response was UTP>=ATP>BzATP>=Adenosine. Mucosal ATP hyperpolarized V_t from -1.1 ±0.3 to -3.7 ±0.4 mV; and reduced transepithelial resistance, R_t, from 385 ±32 to 185 ±16 W•cm² (n=15). Similarly, serosal ATP changed V_t from -1.5 ±0.2 to -4.7 ±0.6 mV and R_t from 408 ±30 to 247 ±18 W•cm² (n=15). Application of the Ca²⁺-activated Cl^- channel blocker niflumic acid (mucosa, 0.1 mM) inhibited 50% of mucosal ATP effects (P < 0.05). In contrast, the effect of serosal ATP was not influenced by mucosal niflumic acid. Conclusion: We conclude that Capan-1 cells grown on permeable support form epithelial monolayer with several types of functional receptors, and thus it is a useful model of human pancreatic ducts. Our data also indicate that mucosal ATP open Ca²⁺- activated Cl^- channel and may stimulate pancreatic duct secretion, while serosal ATP regulates secretion in a different way.