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Acta Physiologica 2009; Volume 197, Supplement 675
Joint meeting of The Slovenian Physiological Society, The Austrian Physiological Society and The Federation of European Physiological Societies
11/12/2009-11/15/2009
Ljubljana, Slovenia
EFFECT OF CALLUNA VULGARIS ON SINGLE DOSE UVB-INDUCED OXIDATIVE STRESS IN SKH-1 MICE SKIN
Abstract number: P235
Filip1 Adriana, Clichici1 Simona, Daicoviciu1 Doina, Tatomir2 Corina, Achim1 Marcela, Postescu2 I.D., Muresan1 Adriana, Moldovan1 Remus
1University of Medicine and Pharmacy Iuliu Hatieganu Cluj Napoca, Romania
2Oncologic Institute Prof. Dr. I. Chiricuta Cluj Napoca, Romania
Skin carcinomas represent approximately 30% of the new cases of diagnosed cancer. Ultraviolet radiation (UV) from the sun is a major cause of non-melanoma skin cancer in humans. The prevention and mainly the photo chemoprevention with natural products represent a simple but very effective strategy in the management of cutaneous neoplasia.
Phenol compounds and triterpenes (ursolic acid and oleanoic acid) isolated from Calluna Vulgaris (Ericaceae family), had proven in vitro remarkable effects in prevention UV-induced photo carcinogenesis and toxicity.
In this study we have investigated the effects of topical application of Calluna Vulgaris (CV) extract in 70% ethanol to skin mice before one dose UVB irradiation on markers of oxidative stress and matrix metalloproteinase's activities (MMPs).
Forty-two 8-week-old female SKH-1 hairless mice were randomly divided into 6 groups: 1. group I (control) - no treatment, no irradiation; 2. group II - treated with 50 mg/cm2 hydrogel vehicle, no irradiation; 3. group III – UVB-irradiated group; 4. group IV – vehicle and UVB irradiated; 5. group V – CV extract treated (4 mg polyphenols/30ml/cm2), UVB irradiated; 6. group VI – CV extract incorporated in vehicle, UVB irradiated. UVB irradiation was performed after 30 minutes from topical application with 240 mJ/cm2. The animals were sacrificed 24 hours after irradiation. Malondialdehyde (MDA) and glutathione (GSH) levels were determined in skin homogenates using fluorimetric methods. MMP-2 and MMP-9 activities were evaluated in skin with zymographic method.
The results indicated that the treatment with CV extract inhibited UVB-induced oxidative stress especially when was incorporated in vehicle (MDA: 0,76±0,04 nmoles/mg protein vs. 2,81±0,94 nmoles/mg protein in irradiated group, p<0,001) or irradiated vehicle group (0,86±0,24 nmoles/mg protein). GSH level increased after irradiation (17,6±7,10 vs. control group 9,3±5,3 nmoles/mg protein) and decreased in animals treated with CV incorporated in vehicle (8,4±1.02 nmoles/mg protein, p<0,05).
MMP-9 and MMP-2 activities in skin slightly increased in vehicle and irradiation group comparatively with CV treated groups.
In conclusion our results demonstrated that the damage effects of one dose of UVB are mediated by generating of reactive oxygen species.
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 197, Supplement 675 :P235