It is well known that a moderate reduction of muscle blood flow (low body positive pressure of 50 mmHg) during endurance continuous exercise training enhances the local aerobic muscle adaptations. The present study investigates the effect of interval training combined with thigh cuffs pressure of +90 mmHg on maximal and submaximal performance. Twenty untrained individuals (age = 23.0 ± 4.2 yrs; stature = 168.3 ± 9.6 cm; body mass = 64.2 ± 15.0 kg) were assigned either to control (CON) or to experimental (CUFF) training group. All participants carried out an incremental exercise test to exhaustion (VO_2max), a 6-min constant test at 80% of VO_2max (Sub₈₀) and a constant power test (TF₁₅₀) pre and post training. Furthermore, they obtained an incremental exercise test with cuffs inflated to external pressure of +90 mmHg (VO_2maxPress) pre-training in order to be determined the training intensity. Both groups performed interval training on cycle-ergometers 3 d·wk^-1 for 6 wks. The CON group trained on cycle ergometer without cuffs, whereas the CUFF group trained with cuffs on thighs pressurized to +90 mmHg (cuffs depressurized during active recovery) at the same relative intensity. In particular, each training session consisted of 2-min work bout at 90% of VO_2max or VO_2maxPress: 2-min active recovery bout at 40% of VO_2max. Despite the unchanged VO_2max, both groups increased significant PPO (CON: Pre = 207.2 ± 60.6 Watts, Post = 237.7 ± 77.2 Watts; CUFF: Pre = 182.3 ± 40.8 Watts, Post = 227.0 ± 37.7 Watts; P<= 0.05) that was accompanied by higher deoxygenation (DStO₂) (CON: Pre = -15.3 ± 1.3%, Post = -29.1 ± 1.3%; CUFF: Pre = -21.6 ± 1.5%, Post = -42.4 ± 1.6%; P<= 0.05) measured with near infrared spectroscopy (NIRS); the deoxygenation was more pronounced on CUFF group (P<= 0.05) at the same relative PPO. Moreover, both groups reduced VO₂ (P<= 0.05) during Sub₈₀ without concomitant changes in DStO₂. Also, CON and CUFF group improved TF₁₅₀ by ~40% and ~32%, respectively; but there were no differences between training groups. It seems that 6-wks interval training combined with thigh cuffs pressure of +90 mmHg on exercised legs at the same relative intensity does not provide any additive effect on maximal and submaximal performance. However, despite the lower absolute training intensity of CUFF group, the enhanced PPO that was accompanied by higher DStO₂ may reveal improvement of peripheral aerobic factors transferring and consuming O₂.