Aim: 

The antiproliferative and proapoptotic effects of two extracts of polyphenols (PO) from wastewater of extra virgin olive oil (Moraiolo and Ogliarola cultivars) were assessed on human leukaemia cells lines HL60 and K562. Parallel studies were also undertaken to evaluate the effects of these compounds on erythroid differentiation in K562 cells.

Methods: 

The effects of PO extracts on the growth of cells were determined following a 48 hours incubation period in presence of various concentrations of these molecules. The effects on cell cycle were assessed by flow cytometric analysis, in cells exposed up to 24 hours to these agents. Apoptosis was determined by fluorescent microscopy.

Results: 

At 30mg/ml, POs from wastewater of Ogliarola cultivar were able to inhibit the cellular growth by 30% in both cell lines whereas proapototic effects and a decrease of S-phase cell cycle entering cells were highlighted in HL60 cell line only.At 30mg/ml, POs from wastewater of Moraiolo cultivar inhibited the growth of K562 and HL60 cell line by 30% and 50% respectively. Similar results on cell cycle and apotosis were observed for both wastewater from Ogliarola and Moraiolo. Finally, both cultivar wastewater did not show to induce erythroid differentiation on K562 cell lines.

Conclusions: 

The data highlight that POs from wastewater of different cultivars exert antiproliferative effects on HL60 and K562 whereas proapoptotic effects were observed only on HL60 cell lines.