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Acta Physiologica Congress

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Acta Physiologica 2009; Volume 195, Supplement 669
The 88th Annual Meeting of The German Physiological Society
3/22/2009-3/25/2009
Giessen, Germany


INFLUENCE OF FOCUSED AND RADIAL SHOCK WAVE TREATMENT ON THE BEHAVIOR OF HUMAN MESENCHYMAL STEM CELLS (MCSS)
Abstract number: P252

Delhasse1 Y., Neuland2 H., Bloch1 W.

1Dept. of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, Kln
2Centre for Extracorporeal Shock Wave Therapy, Kronberg i. T.

Introduction: 

Recent studies demonstrate the successful use of shock wave therapies for improvement of tissue repair and regeneration; processes where mesenchymal stem cells are involved e.g. wound healing and bone repair. Therefore the question arises as to whether shock waves can influence stem cells involved in tissue regeneration. MSC dependent regeneration can be improved by inducement migration, proliferation and apoptosis. Due to the fact that two different kinds of shock waves (focused and radial) improve mesenchymal stem cell dependent regenerative processes, it seems appropriate to investigate the influence of both kinds of shock waves on MSC.

Methods: 

The first experiment with treatment conditions where the shock waves are reflected by culture dishes shows a dose dependent increase of MSC migration by shock wave treatment. We established a new experimental cell culture setup for shock wave treatment under more absorbing conditions to better simulate in vivo circumstances. We tested the effects of different intensities of energy, frequency and total number of crushes on MSC to investigate migration, proliferation and apoptosis with both kinds of shock waves.

Results: 

We developed methods for in vitro treatments of MSC with both kinds of shock waves with guarantee of cell vitality, which allow investigations of both kinds of shock wave treatments. We could show significant findings in influence of proliferation, apoptosis and migration with both kinds of shock wave treatments. The different shock wave systems had exhibited a different range of efficiency in our investigations. In comparison to focussed shock wave treatment we could not detect any negative effects of migration with radial shock wave treatment.

Conclusion: 

The present results indicate significantly that MSC can be dose dependently influenced by shock wave treatments. We could verified explicit influences of MSCs with both kinds of shock wave but with different ranges of efficiency.

To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 669 :P252

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