Acta Physiologica 2009; Volume 195, Supplement 669
The 88th Annual Meeting of The German Physiological Society
HYPERALDOSTERONISM IN KLOTHO-DEFICIENT MICE
Abstract number: P236
Fischer1 S. S., Kempe1 D. S., Rexhepaj1 R., Rosenblatt2 K., Kuro-o3 M., Lang1 F.
1Department of Physiology, Eberhard-Karls-University of Tbingen, Tbingen
2Dept. of Biochem. & Mol. Biol., University of Texas, Galveston, United States of America
3Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States of America
Klotho inhibits 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) formation. Klotho-deficient mice (klotho-/-) suffer from severe growth deficit, rapid ageing and early death, events largely reversed by vitamin D deficient diet. The present study explored the role of Klotho-deficiency on mineral and electrolyte metabolism. To this end, klotho-/- mice and their wild type littermates (klotho+/+) were subjected to normal diet (D+), Vitamin D deficient (D-) diet or to vitamin D deficient diet for 4 weeks and then to normal diet (D-/+). At the age of 8 weeks, body weight was significantly smaller in klotho-/-D+ mice than in klotho+/+D+ mice, klotho-/-D- and klotho-/-D-/+ mice. Plasma concentrations of 1,25(OH)2D3 and aldosterone were significantly higher, plasma PTH concentrations significantly lower in klotho-/-D+ mice and klotho-/-D-/+ mice than in klotho+/+D+ mice and klotho-/-D- mice. Plasma Ca2+, phosphate and bicarbonate concentrations tended to be higher and plasma K+ concentration tended to be lower in klotho-/-D+ mice and klotho-/-D-/+ mice than in klotho+/+D+ mice and klotho-/-D- mice. Urinary excretion of Ca2+, phosphate and K+ was significantly higher in klotho-/-D-/+ mice than in klotho-/-D- and in klotho+/+D+ mice. In conclusion, the present observation disclose that the excessive formation of 1,25(OH)2D3 in Klotho-deficient mice affects not only mineral but as well electrolyte metabolism.
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Acta Physiologica 2009; Volume 195, Supplement 669 :P236