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Acta Physiologica 2009; Volume 195, Supplement 667
XXXV Congress of The Spanish Society for Physiological Sciences
2/17/2009-2/20/2009
Valencia, Spain
ACC AND AMPK DISSOCIATION DOES NOT SEEM TO BE MEDIATED NEITHER BY ERK1/2 NOR P38MAPK
Abstract number: P25
Ponce-Gonzalez1 JG, Guillen-Salgado1 J, Guadalupe-Grau1 A, Fuentes1 T, Olmedillas1 H, Santana1,2,3 A, Morales-Alamo1 D, Calbet1 JAL, Guerra1 B
1Department of Physical Education, University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Canary Islands, Spain.
2Genetic Unit, Childhood Hospital-Insular-Materno Infantil de Las Palmas, Las Palmas de Gran Canaria, Canary Islands, Spain.
3Research Unit, Hospital de Gran Canaria Dr. Negrn, Bco Ballena s/n, Las Palmas de Gran Canaria, 35013, Canary Islands, Spain. [email protected]
Aim:
To study the possible dissociation between AMPK activity and ACC phosphorylation immediately after a sprint exercise in human skeletal muscle.
Methods:
Ser221-ACCb, Thr172-AMPKa, Thr202/Tyr204-ERK1/2, Thr180/Tyr182-p38 MAPK phosphorylation levels in skeletal muscle were determined by immunoblotting in fifteen young healthy men in response to a 30 s sprint exercise (Wingate test). Subjects were randomly distributed into two groups. One group exercised under fasting conditions (n=7, C), and the other ingested 75g of glucose (G) one hour before exercising (n=8).
Results:
Ser221-ACC phosphorylation was increased immediately after the Wingate test and 30 min into the recovery period. Ser221-ACC phosphorylation level detected after sprint exercise was unaffected by the glucose ingestion. However, Thr172-AMPKa phosphorylation was increased 30 min after the sprint exercise in C, whereas was blunted in G. Thr202/Tyr204-ERK1/2 phosphorylation was unchanged immediately following and increased 30 min after the Wingate test in C. Thr180/Tyr182-p38 MAPK phosphorylation level was unaffected by the sprint exercise in C. Glucose ingestion blunted Thr202/Ty204-ERK1/2 phosphorylation 30 min after the Wingate test and increased basal Thr180/Tyr182-p38 MAPK phosphorylation level.
Conclusion:
Sprint exercise elicits an immediate Ser221-ACCb phosphorylation in human skeletal muscle. This Ser221-ACCb phosphorylation increase is maintained during the first 30 min of the recovery period after the Wingate test and is independent on the glucose ingestion. We provide evidence that this phosphorylation does not seem to be mediated by AMPK, ERK1/2 or p38 MAPK activation in human skeletal muscle.
Granted by the Ministerio de Educación y Ciencia (BFI2003-09638, BFU2006-13784 and FEDER) and the Gobierno de Canarias (PI2005/177).
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 667 :P25