Visfatin is a novel member of the adipokine family and is identical to nicotinamide phosphoribosyltransferase (Nampt), an essential rate-limiting enzyme in biosynthesis of nicotinamide adenine dinucleotide (NAD⁺). Regulation of visfatin remains largely unknown. To study the regulation of visfatin in response to nutritional state, we examined expression levels of visfatin mRNA in fasted and fed mice. 3-month-old male C57BL/6J mice were fed ad libitum, or fasted 18 hrs and sacrificed. The mRNA expression was analyzed by real-time PCR and protein levels were assessed by Western blotting. Visfatin mRNA in brown adipose tissue (BAT) was 10-fold higher compared to liver (p<= 0.001) and 2,5-fold higher than in visceral adipose tissue (vWAT). Visfatin mRNA was 2-fold higher in vWAT than in subcutaneous (scWAT). The expression of visfatin in BAT was confirmed on protein level (4,8-fold higher compared to liver, p<=0.01). Visfatin mRNA in BAT and liver was significantly increased by 2-fold and 1.6 fold (p<=0.01 in both), respectively, after 18 hrs fasting, while mRNA in vWAT or scWAT did not change compared to fed mice. Visfatin is highly expressed in brown adipose tissue in mRNA and protein levels and visfatin mRNA is upregulated in liver and BAT by fasting. As visfatin is a regulator of NAD+ synthesis, the expression of visfatin in BAT might suggest a novel role of visfatin in the regulation of energy metabolism and thermogenesis.