Acta Physiologica 2007; Volume 189, Supplement 653
The 86th Annual Meeting of The German Physiological Society
ARISED INCREASE OF THE INTRACELLULAR CALCIUM CONCENTRATION BY CO-APPLICATION OF CHEMOTHERAPEUTICS
Abstract number: P15-L4-12
Gunes1 DA, Florea1 AM, Splettstoesser1 F, Busselberg1 D
1Universittsklinikum Essen, Institut fr Physiologie
Induction of apoptosis in specific cell-lines is an important clinical aspect, that could be triggered by an increase of the intracellular calcium concentration ([Ca2+ ]i). We have shown that arsenic trioxide (As2O3) and cisplatin (CDDP) elevate [Ca2+ ]i by two different mechanisms (As2O3: depletion of intracellular Ca2+ stores; CDDP: influx). The intention of this study was to proove: A) independency of these effects and B) possible synergistic effects triggered by co-applicaton.
As2O3 and/or CDDP were single-/co-applied in human SY-5Y neuroblastoma cells and in mouse hippocampus cells (MHC) (1mM for 60 min. by flow-system at room-temperature). Cells were loaded with Fluo-4 and measurements were taken by confocal laser scanning microscope.
Application of these substances triggers different calcium signals: A) sustained increase and B) transient elevations. Single- appication of As2O3/CDDP induces a sustained-increase of 37±31%/42±15% (SY-5Y) and 74±50%/21±20% (MHC) while co-application of CDDP to As2O3 or As2O3 to CDDP increases [Ca2+ ]i by 70±57%/114±25% (SY-5Y) or 324±198%/137±113% (MHC). Number of fast transient elevations was increased by 40%/30% (As2O3), 74%/36% (CDDP) and 60%/80% (As2O3 + CDDP) (SY-5Y/MHC).
The use of such synergistic effects could possibly increase the efficiency of chemotherapy and reduce side-effects.
To cite this abstract, please use the following information:
Acta Physiologica 2007; Volume 189, Supplement 653 :P15-L4-12