Nogo-A mRNA and protein expression has been found in myelin and neurons in the CNS. The inhibitory function of Nogo-A in myelin in the adult CNS during regeneration has been well documented but nothing is known about the role of Nogo-A in neurons. In cerebellar Purkinje neurons, Nogo-A expression is relatively high during development, but downregulated in the adult. We have generated transgenic mice which overexpress the neurite growth inhibitory protein Nogo-A in cerebellar Purkinje cells under the control of the L7 promoter. With age, transgenic mice started to develop dyskinesia. These findings suggest the hypothesis that Nogo proteins may play a role in cerebellar physiology. To investigate this question, current clamp recordings have been performed from Purkinje cells in cerebellar slices of wild type mice and mice overexpressing NOGO-A using the perforated patch configuration of the patch clamp technique. We observe an alteration the intrinsic electrophysiological properties of the Purkinje cells from mice overexpressing NOGO-A. Further studies are needed in order to provide a link between the observed increased excitability of Purkinje cells and the selective overexpression of NOGO-A protein.