Arthritis & Rheumatism, Volume 63,
November 2011 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Chicago, Illinois November 4-9, 2011.
Correlations Between S100 Gene Expression Levels and the Local and Systemic Inflammatory Markers (matrix metalloproteinase-3, MMP3; erythrocyte sedimentation rate, ESR) in Rheumatoid Arthritis Patients.
Lee1, Hooi-Ming, Sugino1, Hidehiko, Aoki2, Chieko, Murakami2, Miho, Matsutani2, Takaji, Ochi3, Takahiro, Nishimoto2, Norihiro
In humans, the S100 protein family is composed of at least 24 members and is thought to be involved in cell proliferation and differentiation via calcium regulation. Overexpressions of several S100 proteins have recently been reported in rheumatoid arthritis (RA). Thus, we performed a comprehensive gene expression analysis in RA patients to investigate the S100 gene expression levels and further investigating the correlations with clinical and laboratory markers.
Gene expression profiles of the peripheral blood from 112 RA patients with active disease (DAS28, 6.2 ± 0.9; CRP, 32 ± 24 mg/l; MMP3, 308.2 ± 225.3 ng/ml; ESR, 51.9 ± 26.1 mm/h; mean ± SD) and 45 healthy individuals (HI) were obtained using DNA microarray. S100 gene expressions were compared between the two groups using unpaired Mann-Whitney test. Clinical and laboratory markers including DAS28, serum MMP3 levels, and ESR of RA patients were obtained and correlations between expression levels of differentially expressed S100 genes and each marker were investigated using Spearman's rank correlation.
The expressions of fifteen S100 genes were investigated. S100A4, S100A5, S100A6, S100A9, S100A11, and S100A12 are significantly overexpressed in RA patients (P <0.0005). S100G were also increased (P <0.005) while S100A2, S100A13, S100A16, and S100B were underexpressed (P <0.05). There were no correlations between the expression levels of differentially expressed S100 genes and DAS28. However, the expression levels of S100A6 and S100A9 were found significantly correlated to MMP3 levels (Spear r = 0.25, 0.24, respectively; P <0.05), which may reflect joint inflammation and it is reported to be valuable for predicting bone damage progression, especially in the early stage of RA, and S100A12 was correlated to ESR, an inflammatory marker (Spear r = 0.21; P <0.05).
We confirmed the previously described up-regulation of S100A4 and S100A12, and also newly found up-regulation of S100A5, S100A6, S100A9, and S100A11 in RA patients' peripheral blood. Correlation of S100A6 /S100A9 expression levels and serum MMP3 levels; S10012 expression levels and ESR suggested their roles in inflammatory conditions in RA.
To cite this abstract, please use the following information:
Lee, Hooi-Ming, Sugino, Hidehiko, Aoki, Chieko, Murakami, Miho, Matsutani, Takaji, Ochi, Takahiro, et al; Correlations Between S100 Gene Expression Levels and the Local and Systemic Inflammatory Markers (matrix metalloproteinase-3, MMP3; erythrocyte sedimentation rate, ESR) in Rheumatoid Arthritis Patients. [abstract]. Arthritis Rheum 2011;63 Suppl 10 :1925