Arthritis & Rheumatism, Volume 63,
November 2011 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Chicago, Illinois November 4-9, 2011.
Synovial Tissue Cytokine Expression in Rheumatoid Arthritis and Associations with Lymphoid Neogenesis, Disease Activity and the European League Against Rheumatism Response.
Canete1, Juan D., Celis2, Raquel, Ramirez1, Julio, Marsal3, Sara, Avila3, Gabriel, Sanmarti2, Raimon, Pablos4, Jose L.
Hospital Clinic, Barcelona, Spain
Clinic Hospital, Barcelona, Spain
Hospital Vall de Hebró, Grup de Recerca de Reumatologia, Barcelona, Spain
Instituto de Investigación Hospital 12 de Octubre (I+12), Madrid, Spain
We analized wether LN is associated with specific patterns of inflammatory cytokine expression in synovial tissue (ST), and the potential association of cytokine expression with disease activity and response to therapy.
ST samples were obtained by arthroscopy from the inflamed knee of 62 RA patients, In twenty-one patients which started TNF blockers a second biopsy was obtained after a mean of 8+5 months of treatment. ST samples were immunostained with CD3 (T cell), CD20 (B cell), and MECA-79 (high endothelial vessels). Total ST mRNA was extracted and gene expression of CCR7, LT-beta, IL-7, IL-10, IL-17A, IL-21, IL-22, IL-23, TNF-alpha, IL-1b, and IL-6 was measured by quantitative real-time PCR. Clinical and biological data were collected at inclusion and after a median of 2.3 years of follow-up.
28 out of 62 patients (45.2%) had LN, which correlated with a significantly-higher expression of three key molecular markers of LN, CCR7, IL-7 and LT-beta (p<0.037, p<0.038, and p=0.009, respectively). LN-positive patients also had higher IL-23 (p=0.035) and higher DAS28 score, both at inclusion and at the end of follow-up (p=0.055 and p=0.037, respectively). In the group of patients with ST before and after TNF blockers, EULAR good response was associated with IL-10 reduction (p=0.035). In the entire group EULAR response correlated with IL-1b expression (P=0.014).
RA patients with LN are characterized by higher expression of key molecular markers of LN, IL-23 and higher disease activity. Significant reduction in IL-10 expression after therapy with TNF-blockers is associated with good EULAR response.
To cite this abstract, please use the following information:
Canete, Juan D., Celis, Raquel, Ramirez, Julio, Marsal, Sara, Avila, Gabriel, Sanmarti, Raimon, et al; Synovial Tissue Cytokine Expression in Rheumatoid Arthritis and Associations with Lymphoid Neogenesis, Disease Activity and the European League Against Rheumatism Response. [abstract]. Arthritis Rheum 2011;63 Suppl 10 :1806