Arthritis & Rheumatism, Volume 63,
November 2011 Abstract Supplement

Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Chicago, Illinois November 4-9, 2011.


Evaluation of Circulating Endothelial and Platelet Microparticles In Men with Ankylosing Spondylitis.

Sari1,  Ismail, Bozkaya2,  Giray, Kirbiyik2,  Halil, Alacacioglu2,  Ahmet, Ates1,  Halil, SOP2,  Gulten, Sen3,  Gercek

Dokuz Eylul University School of Medicine, Izmir, Turkey
Izmir Bozyaka Training and Research Hospital, Izmir, Turkey
Izmir Ataturk Training and Research Hospital, Izmir, Turkey
Izmir Tepecik Training and Research Hospital, Izmir, Turkey

Background/Purpose:

Microparticles (MPs) are molecules that are released from the various cells, most notably from platelets and endothelial cells, during the activation or apoptosis. MPs are involved in a wide range of physiological and pathological processes. MPs may modulate endothelial function by stimulating cytokine release, intervening in the production of nitric oxide and prostacyclin by endothelial cells as well as monocyte chemotaxis and adherence to the endothelium. Endothelial dysfunction has increasingly been studied in patients with ankylosing spondylitis (AS) with yet no clear mechanism to explain. In the present study, we aimed to evaluate the endothelial microparticle (EMP) and platelet microparticle (PMP) profiles in men with AS and healthy subjects to test whether PMPs and EMPs are altered in AS. We also aimed to determine whether the concentrations of MPs correlate with disease activity (BASDAI), function (BASFI), and spinal mobility (BASMI).

Methods:

This descriptive, cross-sectional study comprised 82 men with AS according to the modified New York criteria and 53 healthy controls. Subjects with a history of chronic diseases including coronary artery disease, hypertension, diabetes mellitus, and dyslipidemia were excluded. MPs were isolated from plasma by ultracentrifugation. Following the isolation, MPs were stained with monoclonal antibodies against platelets, and endothelial cells, and quantified by using flow cytometry. MPs which are positive for both (CD31+/CD42+) and total CD42(+) are identified as PMPs; and MPs consisting (CD31+/CD42-), and total CD144(+) are considered as EMPs.

Results:

Age, waist circumference, smoking status, fasting glucose and serum lipids were similar between the patient and control groups (p>0.05, Table 1). Mean disease duration was 13.2±8.9 years. There were no differences in regard with the concentration of EMPs and PMPs between the AS patients and healthy controls (p>0.05, Table 1). The comparison of active (BASDAI>=4) and inactive disease state AS patients showed that the concentration of EMPs and PMPs were not different between the groups (p>0.05). Correlation analysis revealed no correlation with BASDAI, BASFI or BASMI. However, that CRP, and HDL were significantly correlated with total CD42(+) PMPs (r=0.5, and -0.3), CD42(+)CD31(+) PMPs (r=0.4, and -0.3), and CD42(-)CD31(+) EMPs (r=0.3, and -0.2). Total CD144(+) EMPs showed significant correlations with fasting glucose and triglycerides (r=0.3 and 0.3).

Table 1. Clinical and laboratory results of the study group

 AS Patient (N=82)Healthy Subjects (N=53)P Value
Age (years)39 (17–63)35 (24–60)0.7
Waist circumference (cm)89 (64–10)92 (62–112)0.6
Fasting glucose (mg/dL)88 (65–120)87 (50–123)0.2
LDL cholesterol (mg/dL)106 (34–157)116 (54–155)0.07
Systolic blood pressure (mmHg)110 (100–140)115 (100–140)0.6
Smoking status (%)40.450.60.3
Platelet microparticles (particle/mL)   
Total CD42(+)7371 (938–230565)8472 (364–22552)0.5
CD42(+)CD31(+)5896 (390–21947)6571 (158–21824)0.7
Endothelial microparticles (particle/mL)   
CD42(-)CD31(+)959 (107–3259)1006 (72–3107)0.07
Total CD144(+)1125 (341–6912)1391 (489–4038)0.07

Conclusion:

Circulating EMPs and PMPs, one of the indicators and mediators of vascular injury, are not significantly altered in male AS patients who do not have classical cardiovascular risk factors.

To cite this abstract, please use the following information:
Sari, Ismail, Bozkaya, Giray, Kirbiyik, Halil, Alacacioglu, Ahmet, Ates, Halil, SOP, Gulten, et al; Evaluation of Circulating Endothelial and Platelet Microparticles In Men with Ankylosing Spondylitis. [abstract]. Arthritis Rheum 2011;63 Suppl 10 :1319
DOI:

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