Arthritis & Rheumatism, Volume 63,
November 2011 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Chicago, Illinois November 4-9, 2011.
Salivary Genomic Biomarkers for Primary Sjgren's Syndrome: Correlation with Minor Salivary Gland Lymphocyte Focus Score and Serum Interleukin-17, 21, 23 Level.
Park1, Sung-Hoon, Kim1, Ji Hun, Kim2, Seong-Kyu, Choe3, Jung-Yoon, Kim4, Sang-Hyon, Kim5, Ji-Yoon
Arthritis and Autoimmunity Research Center, Catholic University of Daegu School of Medicine, Daegu, South Korea
Arthritis and Autoimmunity Research Center, Catholic University of Daegu School of Medicine, Daegue, South Korea
Arthritis and Autoimmunity Research Center, Catholic university of Daegu, School of mediine, Daegu
Dongsan Medical Center, Keimyung University, Daegu, South Korea
Dongsan Medical Center, Keimyung University, School of medicine, Daegu, South Korea
Background/Purpose:
To evaluate the expression level of salivary FCGR3B, GBP-2, GAPDH mRNA in primary Sjögren's syndrome(pSS) and to investigate its correlation with minor salivary gland lymph focus score(LFS) and serum Interleukin(IL)-17, 21, 23 Level.
Methods:
Saliva samples were collected from 8 female patient with pSS(age = 38 ~ 55, mean ± SD = 46.13±6.01) and healthy female controls(age = 33 ~ 45, mean ± SD = 39.75 ± 5.12) using our standardized saliva collection protocols for comparative analysis. Subject should refrain from eating, drinking, or oral hygiene procedures for at least 1 hour prior to collection. After collection, the saliva samples were immediately mixed with RNAprotect Saliva Reagent(Qiagen, Hilden, Germany) to the RNA in the saliva sample was stabilized and then centrifuged at 10,000g for 10 minutes. The supernatant was removed from the pellet, immediately aliquoted, and stored at -80°C. cDNA was synthesized with oligo-d(T), Improm- II TM 5 × reaction buffer, 25mM MgCl2, 10mM dNTP, Improm-II TM reverse transcriptase, 25°C 5min, 42°C 60min, 70°C 15min. cDNAs were analyzed by real time quantitative PCR(RT-PCR) in a Finnzymes DyNAmoTMSYBR green qPCR kit (Finnzymes, Beverly, MA, USA). The primer sequences were FCGR3B forward primer, 5`-CAG TGG TTT CAC AAT GTG AA-3`; FCGR3B reverse primer, 5`-ATG GAC TTC TAG CTG CAC-3`, GBP-2 forward primer, 5`-GGA TAT ATT TGG CCC TTT AGA AGA A-3`; GBP-2 reverse primer, 5`-CTT TTT CCT TTT CTG AGA GTG ACT G-3`, GAPDH forward primer 5`-GAA GGT GAA GGT CGG AGT-3`; GAPDH reverse primer, 5`-GAA GAT GGT GAT GGG ATT TC-3`. The results were analyzed with LightCycler software (Bio-rad).
Results:
There was no statistical difference in age between patients and healthy control group. In pSS group, GBP-2 mRNA level was 16.36±35.25 and was significantly correlated with serum IL17 level(94.54±254.65pg/ml, p=0.004) and IL23 level(16.24±22.56pg/ml, p=0.005) by Spearman's correlation. FCGR3B mRNA level and LFS(3.25±2.55) was not correlated with serum cytokine level. FCGR3B mRNA level was significantly correlated with the patient's age(p=0.003). GAPDH mRNA level was not detectable in the patient group.
Conclusion:
GBP-2 mRNA level in saliva of pSS patients was significantly correlated with serum IL17 and IL23 level. Pathophysiologic relevance and possibility of genomic biomarker should be investigated in larger population in the future.
To cite this abstract, please use the following information:
Park, Sung-Hoon, Kim, Ji Hun, Kim, Seong-Kyu, Choe, Jung-Yoon, Kim, Sang-Hyon, Kim, Ji-Yoon; Salivary Genomic Biomarkers for Primary Sjgren's Syndrome: Correlation with Minor Salivary Gland Lymphocyte Focus Score and Serum Interleukin-17, 21, 23 Level. [abstract]. Arthritis Rheum 2011;63 Suppl 10 :484
DOI:
